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Acta Veterinaria Hungarica
Authors: Vasile-Ioan Muntean, Orsolya Sárpataki, Adrian-Valentin Potârniche, Hemza Meghzili, Bogdan Sevastre and Ioan Marcus

In bovine medicine, blood transfusion practice represents an emergency therapy where time is critical. The aim of this study was to develop a mathematical model to calculate the required blood for transfusion, using parameters which could be easily determined on the spot. Twenty Holstein calves were assigned to two groups depending on body weight (100.1 ± 3.1 kg and 151.1 ± 3.0 kg, respectively). All animals were subjected to an anaemia induction protocol in isovolaemic conditions in order to reduce the packed cell volume (PCV) by more than one third. Twenty-four hours later, each group received an auto-transfusion therapy. In order to find a valid constant for specific weight categories with high confidence interval in cattle, we calculated the value of constant ‘CC’ for each individual, using a formula described for carnivores as a basis. The value of the constant for the 100.1 ± 3.1 kg and 151.1 ± 3.0 kg groups was 80.6315 ± 1.1069 and 76.5294 ± 2.5640, respectively. The comparison between the two groups demonstrated significantly different mean values (P = 0.0002, by t-test) and, furthermore, significantly different values of the constant distributions (P = 0.0001, by the Kolmogorov–Smirnov test).

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Acta Biologica Hungarica
Authors: Roxana Stan, Adriana Hangan, Lucia Dican, B. Sevastre, Daniela Hanganu, C. Catoi, Orsolya Sarpataki and Corina Ionescu

Viscum album L. (Santalaceae) (VA) — a parasitic plant that grows on various trees — has proved a significant anticancer effect in both experimental studies and clinical trials. The present study assesses the influence of oxidative stress in mistletoe induced cytotoxicity in tumor cells, in relation to classic cytostatic therapy. VA ethanolic extract was administered alone and combined with doxorubicin (chloride) in Swiss female mice previously intraperitoneally (i.p.) inoculated with Ehrlich tumor cells (1 × 106/animal) that consequently developed Ehrlich ascites carcinoma (EAC). The administered doses were of 50 mg/kg on the 1st, 3rd and 6th day for the VA extract, respectively of 2.5 mg/kg on the 1st and 6th day for doxorubicin, after tumor cell implantation. Fourteen days later all mice were euthanized, ascites of the EAC were collected in order to analyze the tumor proliferation parameters, as well as blood samples, in order to evaluate the antioxidant status in plasma. Tumor development was associated with increased activity of plasma enzymes; classic doxorubicin therapy not only prevents the accumulation of ascitic fluid, but also significantly reduces the activity of plasma antioxidant enzymes. Furthermore, in association with VA extract, the protective effect is improved. Oxidative changes in Ehrlich tumor cells consisted in decreased catalase activity and amplified xanthine oxidase and peroxidase activities.

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