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Overpressured layer chromatography (OPLC), ensuring pumpforced constant mobile phase flow and the possibility of overrun, offers the expanded exploitation of fine-particle adsorbent layers for a longer development distance. Using an infusion—transfusion OPLC method with a 26-cm long development, the separation of clove, rosemary, eucalyptus, tea tree, spearmint, thyme, and cinnamon bark essential oil components was achieved with good resolutions. In the combination of OPLC and Aliivibrio fischeri assay, the main essential oil components eugenol, borneol, (−)-R-carvone, thymol, and trans-cinnamaldehyde exhibited antibacterial effect. The OPLC—2,2-diphenyl-1-picrylhydrazyl (DPPH*) test showed two antioxidant components: eugenol and thymol.

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In this study, the antibacterial profiling of the ethanolic leaf extract of greater burdock (Arctium lappa L.) is demonstrated, applying thin-layer chromatography (TLC) coupled bioassays against the Gram-positive soil bacterium Bacillus subtilis and the Gram-negative pepper pathogen Pseudomonas syringae pv. maculicola. The main active component was isolated by eluting from the adsorbent bed and subjected to a targeted characterization by high-performance liquid chromatography–diode array detection–electrospray ionisation–mass spectrometry. The identification of the germacranolide sesquiterpene lactone onopordopicrin was based on its retardation factor, bioactivity in TLC-based methods, and retention tim as well as ultraviolet (UV) and mass spectra, compared to those of the reference substance isolated earlier in our laboratory from Onopordum acanthium leaf.

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Acta Phytopathologica et Entomologica Hungarica
Authors:
Dominika Bodnár
,
Orsolya Viczián
,
András Juhász
,
József Fodor
,
Péter G. Ott
, and
Emese Mergenthaler

Abstract

Some jumping plant-lice species are economically important due to their capacity as vectors of phytopathogenic bacteria, for example phytoplasmas. Previous studies have identified 80 jumping plant-louse species from Hungary; however, little is known about their occurrence during winter. To extend our knowledge of overwintering sites of jumping plant-lice in Hungary, we sampled them from conifers in various regions of the country. One of our main objectives was to find Cacopsylla pruni (Scopoli, 1763), the vector of ‘Candidatus Phytoplasma prunorum' during winter.

The period of this study extended from 2014 to 2020 in the winter months. Insects were collected at 18 sampling sites from Borsod-Abaúj-Zemplén county to Somogy county, located at Alsótekeres, Balatonvilágos, Boldogkőváralja, Budakeszi, Fenyőfő, Gyöngyöspata, Kecskemét, Martonvásár, Mátra Mountain, Nagykovácsi, Nagyszakácsi, Páty, Piliscsaba, Somogytúr, Soroksár, Sóskút and Verpelét.

A total of 1,600 jumping plant-louse specimens belonging to 20 species and three families (Psyllidae, Aphalaridae and Triozidae) were collected and identified during the study. In the case of plum psyllid (C. pruni) four shelter sites were identified as new records for Hungary.

The most common species were Trioza remota, Cacopsylla melanoneura, Trioza urticae, Bactericera albiventris, C. pruni and Cacopsylla crataegi.

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