Authors:Margit Pál, Kata Varga, Olga Nagy, and P. Deák
The anaphase promoting complex or cyclosome (APC/C) is a large protein complex with an ubiquitin ligase activity which specifically targets mitotic regulatory proteins for proteasomal degradation. The APC/C contains at least 11 subunits, most of which are evolutionarily conserved from yeasts to humans. We have isolated and characterized mutant alleles of the gene that codes for the APC10/Doc1 subunit of the
APC/C. Loss of function
mutants have rudimentary imaginal discs and arrest their development as prepupae. Larval neuroblasts from these mutants show gross mitotic defects including high mitotic index, chromosome overcondensation, metaphase-like arrest and frequent aneuploid and polyploid cells. Mitotically arrested cells accumulate one of the main substrates of APC/C, cyclin B, most likely due to disabled ubiquitination activity. Our results suggest that the Apc10/Doc1 subunit has an essential role in establishing E3 ubiquitin ligase activity of APC/C in
Authors:I. Tamáska, Z. Vértesy, A. Deák, P. Petrik, K. Kertész, and László Biró
Bioinspired 1+2D nanoarchitectures inspired by the quasi-ordered structures occurring in photonic nano-architectures of biological origin, like for example butterfly scales, were produced by depositing a layer of SiO2 nanospheres (156 nm and 292 nm in diameter) on Si wafers, over which a regular multilayer composed from three alternating layers of SiO2 and TiO2 was deposited by physical vapor deposition. Flat multilayers were deposited in the same run on oxidized Si (324 nm SiO2 thickness) for comparison. Different types of disorder (in plane and out of plane) were purposefully allowed in the 1+2D nanoarchitectures. The positions of the specular reflection maxima for the flat multilayer and for the two different bioinspired nanoarchitectures were found to be similar. Additionally to this, the bioinspired nanoarchitectures exhibited angle independent diffuse reflection too, which was absent in the flat multilayer. Different model calculations were made to explain the specular and diffuse optical properties of the samples. Satisfactory agreement was obtained between experimental data and model calculations.
Authors:Enikő Vida, Orsolya Valkó, A. Kelemen, P. Török, B. Deák, T. Miglécz, Sz. Lengyel, and B. Tóthmérész
We studied the early vegetation dynamics in former croplands (sunflower and cereal fields) sown with a low-diversity seed mixture (composed of 2 native grass species) in Egyek-Pusztakócs, Hortobágy National Park, East-Hungary. The percentage cover of vascular plants was recorded in 4 permanent plots per field on 7 restored fields between 2006 and 2009. Ten aboveground biomass samples per field were also collected in June in each year. We addressed two questions: (i) How do seed sowing and annual mowing affect the species richness, biomass and cover of weeds? (ii) How fast does the cover of sown grasses develop after seed sowing? Weedy species were characteristic in the first year after sowing. In the second and third year their cover and species richness decreased. From the second year onwards the cover of perennial grasses increased. Spontaneously immigrating species characteristic to the reference grasslands were also detected with low cover scores. Short-lived weeds were suppressed as their cover and biomass significantly decreased during the study. The amount of litter and sown grass biomass increased progressively. However, perennial weed cover, especially the cover of Cirsium arvense increased substantially. Our results suggest that grassland vegetation can be recovered by sowing low diversity mixtures followed up by yearly mowing. Suppression of perennial weed cover needs more frequent mowing (multiple times a year) or grazing.
Authors:László Piros, P. Á. Deák, G. Dallos, Zs. Máthé, and A. Doros
Ureteric complications following renal transplantation are well known to cause significant morbidity and compromised graft survival. The necrosis of a major part of the ureter could be a highly detrimental situation, and hardly solvable complication, that poses great challenges. Herein we are presenting a case report to introduce a possible surgical solution following repeated ineffective radiological interventions, in a patient with ureteric necrosis that appeared 3 months after cadaveric kidney transplantation. We transplanted the right kidney to the right iliac fossa performing end-to-side vascular anastomoses and end-to-side uretero-ureterostomy. His clinical course was uneventful during 3 months, when he presented a mild borderline acute cellular rejection together with dilatation of the pyelon. Percutaneous nephrostomy was performed by interventional radiologist. During further radiologic interventions the stenosis was not permeable. We finally made up our minds for surgical solution. We found a totally necrotized graftureter. During a second operation we performed a right nephrectomy, transsecting the pyelon. After mobilization of the transplanted kidney approaching and identifying the pyelon, a large pyelopyelar anastomosis was performed with stenting. The postoperative follow-up showed excellent urine flow from the kidney to the bladder, then the TRD was removed. Surgery had to be considered only if minimally invasive procedures are infeasible or ineffective. A regimen of reconstructive methods are well-known, but all cases have to be evaluated individually. If the native kidneys can be removed, their pyelons and entire ureters should be used for reconstruction.
Authors:Veronika Kozma, Gy. Végső, P. Á. Deák, E. Hartmann, A. Németh, Sz. Török, R. Langer, and A. Doros
Kidney neoplasms can occur after kidney transplantation in low percentage. In this report we delineate a rare case of neoplasm in the transplanted kidney detected on screening ultrasonographic examination. Due to the intercalyceal location of the tumor percutaneous radiofrequency ablation was planned with continuous cooling the collecting system avoiding the thermal damage. To the best of our knowledge this method has never been reported applying in transplanted kidney. The two-month CT follow-up verified no residual tumor and the kidney function remained in normal range during this period. These facts imply that the method can be safely applied.
Authors:A. Doros, B. Nemes, Z. Máthé, A. Németh, E. Hartmann, Á. P. Deák, Zs. F. Lénárd, D. Görög, I. Fehérvári, Zs. Gerlei, J. Fazakas, Sz. Tóth, and L. Kóbori
Hepatic artery complication represents recognized sequel of liver transplantation that carries significant morbidity and mortality. Besides retransplantation, hepatic artery recanalization is provided surgically, or by percutaneous angioplasty and stent placement. This study provides an analysis of a single center experience comparing surgical and interventional treatments in cases of early hepatic artery complications.
In this retrospective single center study, 25 of 365 liver transplant recipients were enrolled who developed early hepatic artery complication after transplantation. Percutaneous intervention was performed in 10 cases, while surgical therapy in 15 cases. Mean follow-up time was not different between the groups (505±377 vs. 706±940 days, respectively).
6 patients in the Intervention Group and 10 patients in the Surgery Group are alive. The retransplantation rate (1 and 3) was lower after interventional procedures, while the development of biliary complications was higher. The mortality rate was higher after operative treatment (2 and 5).
Interventional therapy is a feasible and safe technique for treatment of early hepatic artery complication after transplantation. Being less invasive it is an invaluable alternative treatment having results comparable to surgical methods.
Authors:E. Hartmann, A. Németh, Gy. Juharosi, Zs. Lénárd, P. Á. Deák, V. Kozma, P. Nagy, Zs. Gerlei, I. Fehérvári, B. Nemes, D. Görög, J. Fazakas, L. Kóbori, and A. Doros
Hepatocellular carcinoma, which has developed in liver cirrhosis is a disease where liver transplantation can provide a cure both for the tumour and the underlying liver damage. However, patients can only be transplanted when the tumour number and size do not exceed the Milan criteria. Tumour ablation methods — such as radiofrequency ablation — can provide a chance to make the patient eligible for transplantation. Among the 416 Hungarian liver transplanted patients there are 6 who had received different types of ablative therapy as bridging therapy in different institutions. On the basis of analysis of the patients' data we created a guideline for the treatment of cirrhotic patients with hepatocellular carcinoma with the aim of developing a uniform Hungarian approach.
Authors:P. Török, T. Miglécz, O. Valkó, K. Tóth, A. Kelemen, Á.-J. Albert, G. Matus, A. Molnár V, E. Ruprecht, L. Papp, B. Deák, O. Horváth, A. Takács, B. Hüse, and B. Tóthmérész
In the present paper we report original thousand-seed weight data for the flora of the Pannonian Basin. Our goal was to demonstrate the usefulness of seed weight databases by analysing seed weight data in relation to social behaviour types and life forms. We specifically asked the following questions: (i) how the seed weights are related to social behaviour type categories; (ii) how the life form of the species influences seed weight differences between respective social behaviour types? Own weight measurements are provided for 1,405 taxa; and for 187 taxa we published seed weight data for the first time: these were mostly endemics, orchids and/or species with Pontic, Caspian or continental distribution. Several taxonomic or functional groups are underrepresented in our database, like aquatic plants, rare arable weeds and sub-Mediterranean species. Problematic taxa, some difficult-to-harvest species or species with low seed production and cultivated adventives are also underrepresented. We found that the plant strategies expressed by social behaviour types were significantly different in terms of seed weights. The lowest seed weight scores were found for natural pioneers, whereas the highest ones were found for adventives and introduced cultivated plants. Short-lived herbaceous species had significantly higher seed weight scores than herbaceous perennials. No significant differences were found between specialists and generalists within the stress tolerant group. We found that short-lived graminoids possess heavier seeds than perennial graminoids, perennial and annual forbs. Naturalness scores were negatively correlated with seed weights. Our findings showed that seed collections and databases are not only for storing plant material and seed weight data, but can be effectively used for understanding ecological trends and testing plant trait-based hypotheses. Even the identified gaps underline the necessity of further seed collection and measurements.
Authors:P. Török, E. Tóth, K. Tóth, O. Valkó, B. Deák, B. Kelbert, P. Bálint, Sz. Radócz, A. Kelemen, J. Sonkoly, T. Miglécz, G. Matus, A. Takács, V. A. Molnár, K. Süveges, L. Papp, L. Papp Jr., Z. Tóth, B. Baktay, G. Málnási Csizmadia, I. Oláh, E. Peti, J. Schellenberger, O. Szalkovszki, R. Kiss, and B. TÓthmérész
For understanding local and regional seed dispersal and plant establishment processes and for considering the ecotypes and other forms of specific variability, hard data of locally or regionally measured traits are necessary. We provided newly measured seed weight data of 193 taxa, out of which 24 taxa had not been represented in the SID, LEDA or BiolFlor databases. Our new measurements and formerly published data of locally collected seed weight records together covers over 70% of the Pannonian flora. However, there is still a considerable lack in seed weight data of taxonomically problematic genera, even though they are represented in the Pannonian flora with a relatively high number of species and/or subspecies (e.g. Sorbus, Rosa, Rubus, Crataegus and Hieracium). Our regional database contains very sporadic data on aquatic plants (including also numerous invasive species reported from Hungary and neighbouring countries) and some rare weeds distributed in the southwestern part of the country. These facts indicate the necessity of further seed collection and measurements.