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  • Author or Editor: P. Lízal x
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In PI466495, a powdery mildew resistance source of wild barley ( Hordeum vulgare ssp. spontaneum ), one gene conferring powdery mildew resistance was identified in the Mla locus. In this paper, the RGH1a gene sequence was used as source for the development of a cleaved amplified polymorphic sequence (CAPS) marker. Co-segregation between this marker and powdery mildew resistance was analysed by specific DNA fragments associated with each allele of the gene using 286 F 2 plants derived from a cross between winter barley ( H. vulgare L.) variety ‘Tiffany’ and PI466495. For the co-dominant marker RGH1aI1a , three fragments, 370 bp, 82 bp and 59 bp in size, were amplified from F 2 plants exhibiting resistance reaction types 0 and 0–1 to powdery mildew; whereas two fragments, 429 bp and 82 bp in size, were amplified in susceptible plants. Simple procedures based on polymerase chain reaction and restriction enzyme digestion allowed for identifying the plants susceptible to powdery mildew ( Blumeria graminis f. sp. hordei ) and plants homozygous or heterozygous for the resistance allele. The RGH1aI1a marker was positioned 0.85 cM to the resistance gene and the efficiency of marker-assisted selection (MAS), evaluated as the probability of crossing-over between the marker and the targeted gene, was 99%. The CAPS marker RGH1aI1a is a valuable candidate for MAS and gene transfer into barley varieties susceptible to powdery mildew.

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Thirteen accessions of wild barley ( Hordeum vulgare spp. spontaneum ) resistant to powdery mildew caused by the fungus Blumeria graminis f. sp. hordei were studied with the aim of determining the number of resistance genes and their allelic relationships to the Mla locus on the short arm of chromosome 1H. In five accessions (PI391130, PI466193, PI466200, PI466495 and PI466510), the resistance was caused by one gene, in seven accessions (PI354949, PI391081, PI466158, PI466197, PI466211, PI466297 and PI466461) by two independent genes and in PI301004 by three independent genes. The type of inheritance of all analysed genes except two was dominant or semi-dominant; only one of two genes in PI391081 and PI466297 was recessive. Allelism tests confirmed that in 10 accessions one gene was allelic with the Mla locus, and in three accessions (PI391081, PI466193 and PI466297) the resistance genes were different from the Mla locus.

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