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  • Author or Editor: P. Martinek x
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Polymerase chain reaction (PCR) was used to identify the allelic composition of Glu-A1, Glu-B1 and Glu-D1 loci that comprise the high-molecular-weight (HMW) glutenin subunits in 15 triticale genotypes. The Glu-A1b allele was detected most frequently (86.7%) at the Glu-A1 locus; the Glu-A1a and Glu-A1c alleles were only found, respectively, in the ‘Pawo’ and ‘Leontino’ varieties. Five allelic combinations were detected at Glu-B1 [Glu-B1-1a+2o (encoding Bx7+By8*), Glu-B1-1a+2s (Bx7+By18*), Glu-B1-1a+2z (Bx7+By20*), Glu-B1-1b+2a (Bx7*+By8) and Glu-B1-1b+2o (Bx7*+By8*)]. The Glu-D1d allele (HMW subunits 5+10) was observed in breeding lines that carried translocated segments of wheat chromosome 1D. Dough mixing results indicated the Glu-D1d allele provided slight improvements in bread-making quality. Three-year results with Glu-B1b+2o/2a encoding subunits Bx7*+By8/ By8* exhibited positive affects in bread-making quality. These subunits were detected in varieties ‘Mungis’ and ‘Pawo’.

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Semi-dwarf varieties in wheat associated with gibberellic acid (GA3)-insensitive height reducing genes have led to significant increases in yield but often fall below this potential because of poor seedling emergence after deep showing. Alternative semi-dwarf genes may have the potential to reduce plant height without compromising early plant growth. In durum wheat, bulk segregant analysis was used to screen microsatellite markers linked with the GA3-sensitive genes Rht14 in cv. Castelporziano, Rht16 in cv. Edmore M1 and Rht18 in cv. Icaro. Molecular marker Xbarc3-6A for Rht14, Rht16 and Rht18 showed significant polymorphic differences among DNA bulks for height classes. The genes Rht14, Rht16 and Rht18 were linked with Xbarc3 (11.7–28.0 cM) on the short arm of chromosome 6A and they appear to be allelic. Semi-dwarf genes on chromosome 6AS may potentially be used in breeding for improved establishment.

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