Authors:G. Koncz, P. Török, M. Papp, G. Matus, and B. Tóthmérész
Intensification of land use in the last few decades resulted in an increased rate of fragmentation of natural forest habitats. With decreased patch size but increased total borderline length the influence of the surroundings also increased. The extent of influence is especially crucial where the forest stands are adjacent to agricultural lands. We studied the vegetation (cover) and seed bank (soil samples, seedling emergence) along adjacent stands of an abandoned vineyard and edge and interior of an oak forest community (Quercetum petraeae-cerris) widespread in Central-Europe, using five transects (16 m2 plots along each transect). We asked the following questions: (i) How do vegetation and seed bank composition differ between the vineyard and forest interior and (ii) which weeds are able to penetrate into the forest herbaceous understorey vegetation and seed banks from the vineyard? In total, 15 phanaerophytes and 147 herbs were detected. Negatively associated with canopy shading, herb cover proved the lowest in the forest inferior. Few weeds and other ruderals recorded in vineyard penetrated into the forest interior. Mean seed density decreased one order of magnitude from the vineyard to the forest interior (from 20,831 to 2,159 seed/m2). The seed banks of the abandoned vineyard and edge and forest interior were dominated by ruderals, but decreasing proportion of weeds was detected from the vineyard to the forest interior. Characteristic forest herbs possessed at most sparse seed banks. Our results suggest that high canopy cover mitigates the negative impact of surrounding weedy vegetation on the forest herb layer. Therefore, the effect of surroundings is detectable mostly in the seed banks. We can assume that the formation of an increased ruderal herb cover can be foreseen if canopy opens, because the local propagule sources of forest species are missing from vegetation and soil seed banks.
Authors:G. Koncz, Mária Papp, P. Török, Zs. Kotroczó, Zs. Krakomperger, G. Matus, and B. Tóthmérész
We studied the potential role of seed bank in the dynamics of the understorey in a turkey oak-sessile oak forest (Querceteum petraeae-cerris) in Hungary. We used long-term records of the herb layer (1973–2006) and the seed bank composition of 2006 to assess the role of seed bank in the regeneration of herb layer. The total cover of herb layer decreased from 22% (1973) to 6% (1988), and remained low (<10%) till 2006; coinciding with the increasing cover of secondary canopy dominated by Acer campestre. We found a low density seed bank (ca. 1300 seeds/m2). Altogether 33 species were germinated from the soil samples. A few generalist weed species composed the majority of seed bank. It was possible to assign a seed bank type for 19 species; 14 species out of 19 was long-term persistent. We found that the characteristic perennial forest herbs and grasses had only sparse seed bank. The Jaccard similarity between vegetation and seed bank was low (<30%). Our results suggest that the continuous establishment of forest herbs are not based on local persistent seed bank; it should be based on vegetative spreading and/or seed rain.
Authors:Enikő Vida, Orsolya Valkó, A. Kelemen, P. Török, B. Deák, T. Miglécz, Sz. Lengyel, and B. Tóthmérész
We studied the early vegetation dynamics in former croplands (sunflower and cereal fields) sown with a low-diversity seed mixture (composed of 2 native grass species) in Egyek-Pusztakócs, Hortobágy National Park, East-Hungary. The percentage cover of vascular plants was recorded in 4 permanent plots per field on 7 restored fields between 2006 and 2009. Ten aboveground biomass samples per field were also collected in June in each year. We addressed two questions: (i) How do seed sowing and annual mowing affect the species richness, biomass and cover of weeds? (ii) How fast does the cover of sown grasses develop after seed sowing? Weedy species were characteristic in the first year after sowing. In the second and third year their cover and species richness decreased. From the second year onwards the cover of perennial grasses increased. Spontaneously immigrating species characteristic to the reference grasslands were also detected with low cover scores. Short-lived weeds were suppressed as their cover and biomass significantly decreased during the study. The amount of litter and sown grass biomass increased progressively. However, perennial weed cover, especially the cover of Cirsium arvense increased substantially. Our results suggest that grassland vegetation can be recovered by sowing low diversity mixtures followed up by yearly mowing. Suppression of perennial weed cover needs more frequent mowing (multiple times a year) or grazing.
Authors:Á. Horváth, P. Sántha, V. Horváth, Nóra Török, I. Nagy, G. Jancsó, Cs. Vágvölgyi, and F. Somogyvári
A new, rapid method is described which permits the genotyping of genetically modified animals from a microlitre volume of whole blood samples via one step polymerase chain reaction amplification. The major advantage of the presented method is the exclusion of a DNA preparation step, which significantly reduces the time expenditure and work load of the genetic testing. Pilot studies indicate, that this method is efficient and applicable also on tissue biopsies and larger amount of blood providing a rapid and reliable new technique over conventional genotyping approaches.
Authors:Henrietta Papp, I. Török, A. Matsumoto, T. Enomoto, S. Matsuyama, and P. Kása
Earlier neurochemical studies suggested that human brain carboxypeptidase B may play a significant role in the degradation of amyloid-β1-42 in the brain. Using an immunohistochemical technique we report here on the neuronal expression and distribution of this enzyme in the segments (CA1a, CA1b and CA1c) of the CA1 subfield and in area CA4 of the hippocampus in normal and Alzheimer's disease brain samples. Its distribution was compared with the appearance of neurofibrillary tangles in the same brain sample. For immunohistochemical localization of carboxypeptidase B, a specific C14-module antibody was applied, together with the Gallyas silver impregnation technique for the demonstration of neurofibrillary tangles. The results revealed that, in the control samples, most of the immunoreactivity appeared in segment CA1a in the pyramidal cells, less in segment CA1b and least in segment CA1c. In the Alzheimer's disease samples, there was no particular immunostaining in the neurons, but, a large number of silver-impregnated degenerated neurons appeared. The results support the suggestion that carboxypeptidase B may play a significant role in elimination of the intracellular accumulation and toxicity of amyloid-β in the human brain and thereby protect the neurons from degeneration.
Authors:Á. Klein, Margit Kulcsár, Virág Krízsik, R. Mátics, P. Rudas, J. Török, and Gy. Huszenicza
The basic patterns of thyroid hormones [thyroxine (T4) and 3,3',5-triiodothyronine (T3)] and the T4 and T3 responses induced by thyrotropin releasing hormone (TRH) are reported in captive female barn owls (Tyto alba) during the non-breeding period. The main findings of the study, conducted on a total of 10 owls, are as follow: (1) The thyroid gland of barn owl can be stimulated by the classical TRH stimulation test. (2) T3 response was much more pronounced both under cold (around 10°C) and warm (around 20°C) conditions, whereas T4 response ranged so widely that we could not point out any significant change in it. (3) Basal T3 plasma level was significantly (p = 0.036) higher in birds exposed to cold temperature, and they responded to TRH treatment with a lower plasma T3 elevation than the birds kept in a warm chamber. This pattern, however, cannot be explained by increased food intake, but is in agreement with the fact that enhanced T3 level may account for higher avUCP mRNA expression, which results in higher heat production on the cell level. From the results it is concluded that altering T3 plasma level plays a significant role in cold-induced thermoregulation.
Authors:L. Hajas, K. A. Scherf, Zs. Bugyi, K. Török, E. Schall, P. Köhler, and S. Tömösközi
In special dietary products for people intolerant to gluten, gluten content is not supposed to exceed the regulatory thresholds. Enzyme-linked immunosorbent assays (ELISAs) are routinely used to quantitate gluten in these products. They measure gliadin/gluten with high specificity and sensitivity, but they have some limitations. Quantitative and qualitative variability of the target proteins among wheat cultivars is a factor that may cause inaccurate results. One of the aims of this work was to characterize the protein composition of five wheat cultivars grown in multiple harvest years and their blends by reversed-phase high-performance liquid chromatography (RP-HPLC). The gliadin/gluten content of these wheat flours was also analysed with two commercial ELISA kits. The effect of differences in protein profiles between the flours from an individual cultivar and the blend of five cultivars, harvest years, as well as processing procedures (dough forming and baking) on the results of two ELISA kits was investigated and their relative magnitude was determined. Among the factors investigated, the differences between flours had the greatest impact on gliadin recoveries.
Authors:Katalin Koczok, Anna V. Oláh, Gabriella P. Szabó, Éva Oláh, Olga Török, and István Balogh
A Smith–Lemli–Opitz-szindróma monogénes, autoszomális recesszív módon öröklődő,
mentális retardációval járó többszörös malformatiós szindróma. A kórkép
kialakulását a koleszterin-bioszintézis utolsó lépését katalizáló enzim, a
7-dehidrokoleszterin-reduktáz defektusa okozza. A szerzők a nemzetközi irodalom
áttekintésével a szindróma patofiziológiájáról, epidemiológiai vonatkozásairól,
klinikai megjelenéséről (tünetek, intellektus, fejlődés, életkori sajátosságok),
diagnosztikájáról és kezeléséről adnak áttekintést. 2004 óta Magyarországon 14
beteg került felismerésre, amely a becsült incidenciaadatok alapján a kórkép
jelentős aluldiagnosztizáltságára utal. A 7-dehidrokoleszterin-reduktáz enzim
elégtelen működése miatt a vérben és a szövetekben alacsony koleszterin- és
magas 7-dehidrokoleszterin-koncentráció mérhető, amely utóbbi kimutatása
szükséges a diagnózis felállításához. Molekuláris genetikai vizsgálattal
lehetséges a kóroki mutációk azonosítása és a praenatalis diagnosztika. A
klinikai kép rendkívül változatos, a leggyakoribb tünet a 2–3. lábujjak
kötőszövetes összenövése. A jelenlegi terápia a koleszterin pótlása, azonban a
legújabb eredmények a 7-dehidrokoleszterinből keletkező oxidatív származékok
kóroki szerepére utalnak, és ez a megfigyelés az antioxidánsok potenciális
terápiás hatékonyságát veti fel. Orv. Hetil., 2015, 156(42),
Authors:Veronika Kozma, Gy. Végső, P. Á. Deák, E. Hartmann, A. Németh, Sz. Török, R. Langer, and A. Doros
Kidney neoplasms can occur after kidney transplantation in low percentage. In this report we delineate a rare case of neoplasm in the transplanted kidney detected on screening ultrasonographic examination. Due to the intercalyceal location of the tumor percutaneous radiofrequency ablation was planned with continuous cooling the collecting system avoiding the thermal damage. To the best of our knowledge this method has never been reported applying in transplanted kidney. The two-month CT follow-up verified no residual tumor and the kidney function remained in normal range during this period. These facts imply that the method can be safely applied.
Authors:Zs. Sári, T. Kovács, T. Csonka, M. Török, É. Sebő, J. Toth, D. Tóth, E. Mikó, B. Kiss, D. Szeőcs, K. Uray, Zs. Karányi, I. Kovács, G. Méhes, P. Árkosy, and P. Bai
Breast cancer is characterized by oncobiosis, the abnormal composition of the microbiome in neoplastic diseases. The biosynthetic capacity of the oncobiotic flora in breast cancer is suppressed, as suggested by metagenomic studies. The microbiome synthesizes a set of cytostatic and antimetastatic metabolites that are downregulated in breast cancer, including cadaverine, a microbiome metabolite with cytostatic properties. We set out to assess how the protein expression of constitutive lysine decarboxylase (LdcC), a key enzyme for cadaverine production, changes in the feces of human breast cancer patients (n = 35). We found that the fecal expression of Escherichia coli LdcC is downregulated in lobular cases as compared to invasive carcinoma of no special type (NST) cases. Lobular breast carcinoma is characterized by low or absent expression of E-cadherin. Fecal E. coli LdcC protein expression is downregulated in E-cadherin negative breast cancer cases as compared to positive ones. Receiver operating characteristic (ROC) analysis of LdcC expression in lobular and NST cases revealed that fecal E. coli LdcC protein expression might have predictive values. These data suggest that the oncobiotic transformation of the microbiome indeed leads to the downregulation of the production of cytostatic and antimetastatic metabolites. In E-cadherin negative lobular carcinoma that has a higher potential for metastasis formation, the protein levels of enzymes producing antimetastatic metabolites are downregulated. This finding represents a new route that renders lobular cases permissive for metastasis formation. Furthermore, our findings underline the role of oncobiosis in regulating metastasis formation in breast cancer.