Authors:F.-Y. Du, X.-H. Xiao, P.-P. Xu, and G.-K. Li
An efficient ionic liquid-based microwave-assisted (IL-MAE) method has been developed for extraction of dehydrocavidine from Corydalis saxicola Bunting (C. saxicola) for subsequent rapid analysis by high-performance liquid chromatography (HPLC). The yield of dehydrocavidine reached 9.446 mg g−1 within 10 min under the optimum IL-MAE conditions (1.5 mol L−1 [hmim]Br as extraction solvent, liquid-to-solid ratio 20:1 (mL:g), and extraction temperature 70°C). Compared with conventional procedures, the proposed IL-MAE method has many advantages, for example high extraction yield, short extraction time, low solvent consumption, no use of volatile organic solvents, and no further sample clean-up before HPLC analysis. The method was validated for limit of detection (LOD) and quantification (LOQ), linearity, precision, recovery, and reproducibility. The calibration range was 5.0–200 mg L−1 and the correlation coefficient, r, was 0.9996. The LOD and LOQ were 0.035 and 0.12 mg L−1, respectively. The relative standard deviations of intra-day and inter-day assays were below 2.6% and 6.5%, respectively. Recovery was between 93.8% and 109.3% with RSD values below 5.0%. The method can be used for rapid and effective extraction and analysis of active components from medicinal plants.
Authors:L. Ma, P. Xiao, J. Cai, X. Li, Z. Ji, Y. Xia, C. Yang, and J. Bao
Uniformity in the height of main stem and tillers is a key factor affecting ideal plant type, a key component in super high-yielding rice breeding. An understanding of the genetic basis of the panicle layer uniformity may thus contribute to breeding varieties with good plant type and high yield. In the present study, a doubled haploid (DH) population, derived from a cross between
rice variety Zhai-Ye-Qing 8 (ZYQ8) and
rice variety Jing-Xi 17 (JX17) was used to analyze quantitative trait loci (QTL) for panicle layer uniformity related traits. Six, four and three QTL were detected for the highest panicle height (HPH), lowest panicle height (LPH) and panicle layer dis-uniformity (PLD), respectively. qHPH-1-1 and qPLD-1 were located at the same interval on chromosome 1. The JX17 allele(s) of these QTL increased HPH and PLD by 2.57 and 1.26 cm, respectively. Similarly, qPLD-7 and qHPH-7 were located at the same interval on chromosome 7, where the ZYQ8 allele(s) increased HPH and PLD by 3.74 and 1.96 cm, respectively. These four QTL were unfavourable for panicle layer uniformity improvement because a decrease of the PLD was accompanied by decrease of the plant height. qPLD-6 and qLPH-6-1 were located at the same interval on chromosome 6, however here the JX17 allele(s) increased LPH, but decreased PLD, suggesting that this QTL was favourable for improvement of panicle layer uniformity. The markers identified in this study are potential for marker assisted breeding for the improvement of the panicle layer uniformity and ideal plant type.
Authors:D. E. Re, V. Coetzee, D. Xiao, D. Buls, B. P. Tiddeman, L. G. Boothroyd, and D. I. Perrett
Experience-dependent changes in mate choice preferences may confer an evolutionary benefit by shifting preferences towards traits that are advantageous for specific environments. Previous studies have demonstrated that prolonged exposure to one type of face biases perceptions of subsequently viewed faces and exposure to one type of body biases perceptions of subsequently viewed bodies. We tested whether preferences in facial adiposity were affected by viewing heavy or light bodies. We first assessed facial adiposity preferences by asking Caucasian participants (n = 59) to transform three-dimensional female Caucasian faces along a body mass index (BMI) continuum until they reached optimal attractiveness. Participants then viewed heavy- or light-bodied two-dimensional images with the faces cropped out before repeating the face preference task. Male and female participants who viewed heavy bodies shifted preferences toward significantly higher facial adiposity, while those who viewed the light bodies showed no significant overall shift. These results provide evidence that adaptation to certain body types affects subsequent preferences for facial adiposity, and suggest that adaptation to one body domain may affect preferences in other body domains.