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  • Author or Editor: Pullela Srinivas x
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Rheum emodi is an important medicinal plant widely used in the Unani system of medicine. A simple, precise and convenient HPTLC method has been developed for analysis of chrysophanol, an antioxidant and a major constituent of the rhizomes of Rheum emodi (Polygonacea) and pharmaceutical formulations of these. This method uses chrysophanol as internal standard. The stationary phase was silica gel 60F 254 , and hexane-ethyl acetate, 45 + 5 ( v/v ) was used as mobile phase. Detection and quantification were performed densitometrically at λ max = 366 nm. The method is characterized by high sensitivity and linearity over a wide concentration range. The results obtained were evaluated statistically.

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The dried fruits of Piper longum are extensively used in Ayurvedic medicinal preparations. A simple and convenient HPTLC method has been developed for standardization of the plant material using the two major constituents, pellitorine and dihydropiperlonguminine, as markers. The stationary phase was silica gel 60 F 254 , hexane-ethyl acetate, 75 + 25 ( v/v ), was used as mobile phase, and detection was at λ = 260 nm. The method is characterized by high sensitivity and linearity over a wide range of concentrations. The results obtained were evaluated statistically.

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Plants of the Hedychium genus are perennial rhizomatus plants belonging to the Zingiberaceae family. Extracts of Zingiberacaea have long been used in traditional medicine. A simple, precise, and convenient HPTLC method has been established for analysis of hedychenone, the major marker compound extracted from the rhizomes of Hedychium spicatum (Buch-Hem). Chromatography was performed on silica gel 60F 254 plates with ethyl acetate-hexane, 20 + 80 ( v/v ), as mobile phase. Detection and quantification were performed densitometrically at λ max = 254 nm with hedychenone as external standard. The method is characterized by high sensitivity and linearity over a wide range of concentrations.

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