Aim of this study is to investigate, by use of the pulse radiolysis technique, the mechanism of oxidation of trypthophan by OH and secondary inorganic radicals. Parallel pulse radiolysis studies were, performed on tryptophan and 1-formyltryptophan. The results seem to indicate that the most important attack on tryptophan residues is an one-electron oxidation. These results are compared with other data from the literature and a general discussion is made.
The paper investigates the effect of glutathione on model biological systems, such as enzymes, (lisozyme, alcoholdehydrogenase,
α-chymotrypsin) and DNA base components. Our results on the inactivation of enzymes and on the chemical changes in DNA base
components show a radioprotective action of glutathione in all systems. The results are discussed in terms of glutathione
free radical reactivity and in the light of current hypothesis on the modification of the radiation damage given by thiol