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- Author or Editor: R. Mani x
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Abstract
A sensitive thyroxin /T4/ radioimmunoassay procedure with a short incubation time /10 min/ similar to a conventional stat test in clinical chemistry is described. The assay parameters such as concentration of antisera and125I-T4, and incubation temperature that affect the kinetics of antigen-antibody reaction have been studied. Within- and between-assay variations were less than 7% CV. Analysis of 45 serum samples by the proposed method and by a conventional assay gave similar results /Y=1.05X–0.187; r=0.990/.
Abstract
Knowledge of the specific activity of the125I-tracer is essential for optimization and for calculation of RIA parameters. The specific activity of the125I-thyroxin used in thyroxin radioimmunoassay /RIA/ has been determined by a simple method involving combination of RIA and displacement analysis. It has been compared with the value obtained by the conventional method based on radioiodination data. Our studies indicate that even for a non-protein hormone like thyroxin the specific activity of125I-thyroxin derived from iodination data is not reliable. The specific activities obtained by displacement analysis were consistent with the experimental findings.
Abstract
Labelling of N-(2,6-diethylacetanilido), N-(p-n-butylacetanilido,N-(p-methoxyacetanilido) and N-acetanilido-iminodiacetic acid analogues with99mTc obtained by MEK extraction has been studied. The acetanilido IDA analogues were synthesized using the condensation reaction between nitrilotriacetic acid anhydride and the corresponding anilines. Conditions are described to label these four HIDA analogues using MEK extracted99mTc with high and reproducible radiochemical purity.
Abstract
113mIn is milked out of a113Sn-113mIn generator with dilute hydrochloric acid for use in nuclear medicine. The concentrations of the various impurities like Sn, Zr and their colloidal forms which may trap113mIn activity have to be initially evaluated before releasing the generator for medicinal use. The authors have evaluated the purity of the113mIn-chloride obtained from the generators in detail. The possibility of using a mixture of HCl and NaCl as an alternative eluent for113mIn has also been investigated. It has been observed that this new eluent gives greater yields of113mIn and renders the final manipulation of isotonicity of indium labelled compounds easy.
Abstract
Solvent extraction separation of99mTc from99Mo using methyl ethyl ketone(MEK) has been found to be an effective method of obtaining99mTc of medicinal purity from low specific activity99Mo. The authors have investigated the effect of alkali and molybdenum concentration on the extraction of99Mo and99mTc into methyl ethyl ketone. The possibility of methyl ethyl ketone forming enol and condensation products and its effect on the final extraction efficiency and purity of99mTc has been studied. Sodium molybdate has been found to have a good salting out effect on99mTc pertechnetate and hence99mTc extraction can be better accomplished from low specific activity99Mo solutions. The ketone seems to form traces of condensation products in the extraction procedure. These have been found to be coextracted with99mTc into MEK but did not affect the extractability of99mTc. It was observed that neutral alumina column removes these condensation products from MEK containing99mTc. Alternately these could be filtered off by acidification of the final aqueous99mTc solution. The studies indicate that under optimum experimental conditions methyl ethyl ketone separates99mTc from99Mo with high efficiency and yields99mTc of high purity suitable for use in nuclear medicine in the form of various labelled compounds.
Abstract
We describe a radioimmunoassay procedure for the measurement of phenytoin (5,5-diphenylhydantoin) in serum samples. Antiserum to phenytoin has been produced against phenytoin-valeric acid-bovine serum albumin conjugate.125I labelled phenytoin-acetic acid-tyrosine methyl ester has been used as a tracer. The assay covers a range of 10–500 ng/cm3 and has a sensitivity of 0.25 ng. The assay is validated by specificity tests, precision profile and recovery tests.
Abstract
A procedure for preparation of a sterile closed system generator for99mTc based on conversion to zirconium molybdate of99Mo produced by neutron activation is reported. The generator is sterilized by autoclaving.99mTc is eluted using 0.9% NaCl with high yield and purity in successive elutions.
Abstract
The authors report here a new approach for making99mTc generators based on neutron irradiation of metallic molybdates and direct elution eliminating intermediate chemical processing steps. This approach tested using zirconium molybdate was found to yield99mTc with good yield and purity. This seems to be the simplest way of making column type99mTc generator even using low flux reactors and merits further detailed evaluation.
Abstract
A solid phase radioimmunoassay for triiodothyronine (T3) has been developed using antibody-immobilized serum albumin microspheres. Antibody albumin microspheres were prepared using a 
spinning disc aerosol generator
. The low density of the antibody-albumin microspheres gives greater mobility for the particles there by ensuring better kinetics to the antigen-antibody reaction. The assay has a single incubation of one hour at 37° C and the separation of the antigen-antibody complex is accomplished by centrifugation. The sensitivity of this assay is 0.3 ng/cm3 and has a range of 0.3–4 ng/cm3.
Abstract
Recent and developing applications of radiotracer techniques are reviewed. Future needs of traaining programmes related to these applications are briefly discussed.
