The functional diversity of native mesorhizobial genotypes nodulating chickpea was assessed using two chickpea cultivars under pot culture conditions. The symbiotic effectiveness ratio of the mesorhizobial genotypes showed the existence of wide functional diversity among the isolates in Haryana soils. Mesorhizobial isolates belonging to the third genotype (MG III) were more efficient than the other genotypes. The majority of the isolates (94%) had intermediate effectiveness and only a very small percentage (2%) were ineffective, while 4–6% of the isolates were highly effective with a symbiotic ratio > 4.0. These included CP2381A, CP741, CP1423 and CP2437. Among all the isolates, strain CP2381A was the most efficient isolate for both cvs HC1 and HC5. The symbiotic ratio of the shoot N contents varied for both the cultivars after inoculation with mesorhizobial isolates from different districts. The most efficient chickpea mesorhizobial isolates were found to be present in soils from the Bhiwani district followed by that from RDS Farm, Hisar, but isolates from Mahendragarh, Fatehabad and Jhajjar were less efficient.