Wheat is the second most important crop after rice in India and occupies approximately 28.5 million hectare area. Salinity is one of the major factors reducing plant growth and productivity worldwide, and affects about 7% of world’s total land area. In India about 6.73 million hectare land area is salt affected. The aim of this study was to investigate the morpho- physiological and biochemical response of wheat to temporal salinity (ECiw = 10.0 dSm–1) exposures. Ten wheat genotypes were evaluated in two successive growing seasons (2012–2014), with complete randomized design with three replications under both salinity stress and non-stress conditions. The morpho-physiological and biochemical character measured in this investigation, inhibited under both salt stresses (S1 & S2) conditions but much more significantly inhibited under long-term salinity exposure (S2) than S1 because interrupting the metabolic process of plant, resulting in reduced growth and productivity. According to correlation result, selection of high yield genotypes can be done based on plant height (0.649*), tiller plant–1 (0.808**) and leaf area (0.687*). The multivariate morphophysiological and biochemical parameters should be further used to develop salinity tolerance in wheat breeding improvement programmes.
Authors:M. R. Sengar, S. V. Gandhi, U. P. Patil, V. S. Rajmane and K. G. Bothara
A simple, specific, accurate, and precise high-performance thin-layer chromatographic method for analysis of cefuroxime axetil and potassium clavulanate in a combined tablet dosage form is reported. The compounds were separated on aluminium foil plates precoated with silica gel 60 F254, with chloroform-methanol-toluene 4:3:3 (v/v) as mobile phase. Densitometric evaluation of the separated bands was performed at 225 nm. The two drugs were satisfactorily separated with RF 0.77 ± 0.0114 and 0.29 ± 0.0114 for cefuroxime axetil and potassium clavulanate, respectively. Response was a linear function of amount over the calibration ranges 500–2500 and 2000–10 000 ng per band, respectively. The method was successfully validated and used for analysis of the drugs in a pharmaceutical formulation. Recovery was 100.05 ± 0.98% for cefuroxime axetil and 99.94 ± 0.538% for potassium clavulanate (mean ± RSD).