Authors:Mhaveer Singh, Yunus Kamal, Ennus Tamboli, Rabea Parveen, Shahid Ansari and Sayeed Ahmad
A novel HPTLC method has been developed for the estimation of glabridin in Licorice rhizome and its Unani polyherbal formulation (Qurs-e-Gul). Separation was achieved on silica using toluene, dichloromethane, and ethyl acetate in equal ratios. A compact, well resolved peak of glabridin with RF value 0.56 ± 0.02 was observed. Calibration curve revealed a good linear relationship with r2 value of 0.993 between the peak area and concentration in the range of 25–500 ng spot−1. The proposed method was validated as per the International Conference on Harmonization (ICH) guidelines. The stability assessment was carried out by studying degradation of glabridin stressed by acid, base, oxidation, thermal, and humidity. Photodegradation was also carried out after keeping the drug in sunlight, dark, and in UV lights. The method proposed can be used for routine determination of glabridin in crude drugs and in herbal formulations containing Licorice as one of the ingredients, for quality control as well as for stability testing with high precision, accuracy and a wide range of linearity.
Authors:Mohammad Tariq, Zeenat Iqbal, Javed Ali, Sanjula Baboota, Rabea Parveen, Mohd. Mirza, Sayeed Ahmad and Jasjeet Sahni
A simple, sensitive, precise, rapid, and reliable high-performance thin-layer chromatographic (HPTLC) method for the simultaneous estimation of sparfloxacin (SPF) and flurbiprofen (FLB) in bulk drug as well as in dual drug loaded poly-(lactic-co-glycolic acid) (PLGA) nanoparticles was developed. In this method, aluminumbacked silica gel 60 F254 plates (20 × 10 cm: 200 μm thickness) were used as stationary phase and chloroform-methanol-formic acid (7.5:1:1, v/v) as an optimized mobile phase. Developed chromatogram was scanned at 258 nm, the wavelength of maximum absorption SPF and FLB. Regression analysis of the calibration data showed an excellent linear relationship between peak area versus drug concentration. Linearity was found to be in the range of 100–600 ng spot−1 and 40–800 ng spot−1 for SPF and FLB, respectively. The suitability of the developed HPTLC method for simultaneous estimation of SPF and FLB was established by validating it as per the ICH guidelines. The limits of detection (LOD) and quantification (LOQ) for SPF were found to be ≈13 and ≈40 ng spot−1, respectively, and those for FLB ≈27 ng spot−1 and ≈82 ng spot−1, respectively. The developed method was found to be linear (r2 = 0.999), precise (% RSD < 1.5% repeatability and <2.55% for intermediate precision), accurate (mean recovery of within the range of 98–102%), specific, and robust. Stress-induced degradation studies revealed the suitability of method for the quantitative determination of drugs in the presence of degradants. The developed method has been successfully applied for the determination of entrapment efficiency, drug loading, in vitro drug release profile and stability assessment of dual drug loaded PLGA nanoparticles (NPs).