Varroa mite, Varroa destructor is the most important ectoparasite of the honey bee, Apis mellifera worldwide, contributing to colony collapse. The development of alternative non-toxic methods against this pest is needed, as most of the wide-used acaricides in apiculture are banned in the European Union, which increase the risk of developing resistant mite populations against the remaining few products. In order to reveal biological basis of a new, semiochemical-based method, the aim of this study was to search for olfactory stimuli, used by female Varroa mites in orienting to drone brood for egglaying. Volatiles of uncapped drone brood were collected in situ, inside bee-hives, using either charcoal, or HayeSep® Q filters. Collections were analyzed by gas chromatograph linked to an electrotarsogram detector (GC-ETD), using the foreleg of female mite. Results showed that most components were present in collections trapped by any of these filters. However, some components appreared only in charcoal-, while others only in HayeSep® collections, respectively. Out of the large number of components, a few elicited electrophysiological responses. Structure elucidation of these active components are underways. Futher behavioral studies should reveal, which components play role in attraction of Varroa mites.