Authors:V. Braga, F. Garcia, J. Dias, and Sílvia Dias
Nb2O5 supported on SiO2-Al2O3 were prepared with a wide loading range (2, 5, 10, 15, 20 and 25 mass%) and analyzed by simultaneous thermogravimetric (TG)
and differential thermal analysis (DTA). The materials presented a phase transition close to 1364°C. This phase transition
was studied by XRD, FTIR and Raman spectroscopy. Amixture of orthorhombic (T) andmonoclinic (H andM) crystalline phases was
evidenced in the supported samples, which is coverage dependent, in contrast to the formation of only the monoclinic phase
(H and M) when pure Nb2O5 is heated under the same conditions. These results indicate the stabilization of Nb2O5 on silica-alumina surface.
Authors:Thiago Henrique M. Vargas, Camila N. Barra, Lidia H. Pulz, Greice C. Huete, Karine G. Cadrobbi, Adriana Tomoko Nishiya, Silvia Regina Kleeb, José Guilherme Xavier, José Luiz Catão-Dias, and Ricardo F. Strefezzi
Mast cell tumour (MCT) is the most frequent skin neoplasm in dogs. These tumours are characterised by variable behaviour and clinical presentation that make prognosis an important and challenging task in the veterinary practice. Galectin-3 (Gal-3) is known to influence several biological processes that are important in the cancer context and has been described as a prognostic marker for several human cancers. The aim of the present work was to characterise Gal-3 immunolabelling in canine cutaneous MCTs and to investigate its value as a prognostic marker for the disease. Thirty-four random cases of canine cutaneous MCT that were surgically treated with wide margins were included in this study. Gal-3 expression was evaluated using immunohistochemistry and the results were compared with the expression of apoptosis-related proteins, Ki67 index, histopathological grades, mortality due to the disease and post-surgical survival. The majority of the MCTs (65.8%) were positive for Gal-3. Gal-3 immunolabelling was variable among the samples (2.7%–86.8% of the neoplastic cells). The protein was located in the cytoplasm or in the cytoplasm and the nucleus. Gal-3 positivity was correlated with BCL2 expression (P < 0.001; r = 0.604), but not with Ki67 and BAX. No significant differences were detected between histological grades or in the survival analysis. Gal-3 expression correlates with BCL2 expression in MCTs. Although an efficient marker for several human neoplasms, the results presented herein suggest that Gal-3 immunolabelling is not an independent prognostic indicator for this disease.