Authors:Tihomira Gojmerac, Marija Uremović, Z. Uremović, S. Ćurić and Nina Bilandžić
The aim of the study was to assess the effect of subacute treatment with a low dose of atrazine (1,3,5-triazine-2,4-diamine, 6-chloro-N-ethyl-N'-(1-methylethyl), an s-triazine herbicide, on endocrine oestrus regulation in gilts. A group of nine gilts (F1generation of Swedish Landrace Ã Large Yorkshire) were treated with 1 mg atrazine/kg body mass daily, mixed to the feed for 19 days before the onset of expected oestrus. Blood samples were obtained by cranial vena cava puncture three times daily at 3-h intervals on five post-treatment days, i.e. before and during oestrus. The serum concentration of oestradiol-17β (E2) was determined by the fluoroimmunochemical method. On Day -2 before the onset of expected oestrus, a significantly lower (P < 0.001) E2concentration was measured in the serum of treated gilts (31.25 ± 1.95 and 39.32 ± 1.38 pg/mL) than in the control pigs (51.43 ± 1.29 and 68.59 ± 2.99 pg/mL). In contrast, the E2concentration measured in the serum of treated animals was significantly higher (P < 0.001) on the day of the expected onset of oestrus and on the subsequent two days (35.43 ± 1.85, 53.92 ± 1.98 and 60.32 ± 2.35 pg/mL, respectively) than in the control animals (13.52 ± 1.79, 21.53 ± 1.35 and 20.05 ± 1.46 pg/mL, respectively). Insufficient serum E2concentration of the treated gilts resulted in a failure of expected oestrus, as indicated also by the state of dioestrus demonstrated by histopathological examination of the uterus.
Authors:A. Svetina, I. Jerković, Ljiljana Vrabac and S. Ćurić
Thyroid function and morphology, liver morphology, some metabolic indices, haematological parameters and growth performance of fattening pigs fed 00-rapeseed meal (00-RPM) were examined. The control group was fed on a standard diet containing 6% sunflower seed meal (SM) during the growing period and 8% during the finishing period. The first experimental group was fed a diet in which SM was replaced by equal (6% and 8%) amounts of 00-RPM. The second experimental group was fed with a higher (8% and 10%) amount of 00-RPM. There were no significant differences between the control and experimental groups in the serum concentrations of triiodothyronine (T3) and thyroxine (T4). Thyroid gland and liver weights were significantly (P < 0.01) higher in both groups fed 00-RPM than in the group fed SM. The epithelium of the thyroid gland was cuboidal or columnar and the follicular area was moderately enlarged in pigs fed 00-RPM. Marked changes in liver histology were not observed. The 00-RPM diet increased (P < 0.01) the serum values of total proteins in the first fattening period. At the end of fattening both groups fed 00-RPM had higher (P < 0.05; P < 0.01) concentrations of plasma glucose than the control group. The inclusion of 10% of 00-RPM during the finishing period increased (P < 0.05) the serum values of insulin. Daily weight gain during the growing and the finishing period was higher (P < 0.05) in the experimental groups than in the control group. The results suggest that 6-10% 00-RPM can be used as a protein source in the diet of fattening pigs without poisonous side effects.