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- Author or Editor: S. Archimandritis x
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Abstract
In the production of131I, the presence of a contamination extractable with organic solvents has been detected and its source discovered. The influence of overheating was established in the preparation of99m Tc by the extraction method. Finally, details are given of the procedure used for the estimation of199Au present in198Au.
Abstract
A procedure for the radiochemical purity control of99mTc-(2,3-dimercaptosuccinic acid) (DMSA), used as a renal scintigraphic agent is described. The proposed chromatographic system entails the use of two successive solvents, first MEK and second aqueous solution of 5% glycine, on the same supporting medium Gelman ITLC-SG. The procedure is fast and leads to separation and estimation of free pertechnetate, hydrolyzed form of99mTc and99mTc — DMSA. This system is superior to the others reported in the literature as the spots of the different species are more distinguished and more concentrated. Its reliability has been studied using dimercaptosuccinic acid kits of different manufacturers and the results have been checked biologically.
Abstract
In this paper chromatographic systems for the accurate and reliable determination of the percentage of different species (free pertechnetate, particulate99mTc and labelled99mTc) in99mTc-labelled radiopharmaceuticals are described. The procedures are based on the simultaneous run of two different chromatograms, one in 85% CH3OH and the other in 15% H3PO4. Also, 0.9% NaCl can be used instead of 15% H3PO4 for the second chromatogram. Alternative procedures applying two dimensional chromatography on Whatman No. 1 paper or silica G plates with 85% CH3OH for the first run and 15% H3PO4 for the second are given. The applicability of these procedures is limited to water-soluble radiopharmaceuticals.
Abstract
The self life of the freeze-dried formulation kits utilized for the preparation of the new bone imaging radiopharmaceutical99mTc-1,2-dihydroxy-1,2-bis/dihydroxyphosphinyl/ethane /99mTc-DHPE/ has been investigated as well as the toxicity of the DHPE. In a biodistribution investigation of99mTc-DHPE in rats, in comparison to99mTc-methylenediphosphanate /99mTc-MDP/,99mTc-DHPE exhibited a certain extent higher skeleton uptake, a higher blood clearance rate, a very low concentration in other organs, a satisfactory biological stability and excretion primarily through the kidneys. These properties demonstrate that99mTc-DHPE is a new promising skeleton imaging radio-pharmaceutical.
Abstract
Quality Control (QC) of radiopharmaceuticals is important, providing products of high standards. The current study refers to an enzymic radioiodination method applied to the labeling of antibodies used in nuclear medicine and to the respective QC procedures. Readily available materials such as125I, polyclonal immunoglobulin G (IgG) and a collection of simple chromatographic techniques helped establish a QC protocol suitable for the radiochemical evaluation of radioiodinated biomolecules. A high pre-purification radiolabeling yield (90%) for total protein was detected by paper electrophoresis (PE) and size exclusion chromatography (SEC), but only the inclusion of SDS-PAGE confirmed the existence of “self-iodination” of lactoperoxidase (LPO) and revealed the true radiolabeling yield for125I-IgG (79%).
