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An interspecies zygote derives after wheat × maize crosses. This zygote aborts in absence of auxin stimulation. Treatment with dicamba is widely used to promote wheat haploid embryo development. We analyzed the effect of dicamba on wheat haploid embryo development. Four treatments related to the time of dicamba treatment were carried out: 1 day before pollination and 1, 5 and 9 days after pollination. It was concluded that wheat haploid embryos developed only in spikes treated with dicamba one or five days after pollination with maize. Dicamba treatment of wheat spikes prior to pollination hinders zygote formation and embryo development. Dicamba treatment 9 days after pollination is too late because young embryos have already aborted.

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Sainfoin ( Onobrychis viciifolia Scop.) is the only species in the genus Onobrychis . Sainfoin is a Eurasian perennial herb that has purple flowers, curved pods and is naturalized in Europe, Asia and North America grasslands on calcareous soil. Sainfoin has been grown in parts of Europe and Asia for hundreds of years. Understanding the relationships among sainfoin ecogeographic, and chemical characteristics may provide insights for better utilizing exotic germplasm. Ten sainfoin populations, were sampled and determined in Serbia and B&H. Some chemical properties, such as crude protein, ash, fat, crude fibre, phosphorus, calcium, magnesium, potassium, K/(Ca+Mg) ratio, and sulfur. Serbia is an important gene origin center for most legume species. Considering the high values of their traits, some Serbian sainfoin populations could serve as a valuable breeding material. In comparison with populations from other parts of the world, Serbian populations are superior in chemical properties and nutritional quality values.

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Cereal Research Communications
Authors: S. Wang, D. Chen, G. Guo, T. Zhang, S. Jiang, X. Shen, D. Perovic, S. Prodanovic, and Y. Yan

In this work, 9 novel LMW-GS genes (6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species were amplified and cloned by allelic-specific PCR. Analysis of the deduced amino acid sequences showed that 7 and 2 LMW-GS had 9 and 7 cysteines, respectively. Four LMW-m type subunits genes had an extra cysteine at the C-terminal III, which could form intermolecular disulphide bonds to extend the chains, and therefore would facilitate to form larger gluten polymers. This suggested that these genes are expected to be used as candidate genes for wheat quality improvement. The correlation between specific N-terminal sequences and a decapeptide deletion in the C-terminal II in LMW-GS encoded by D genome was found. Particularly, if LMW-GS possessed a METRCIPG-N-terminal beginning sequences and a decapeptide (LGQCSFQQPQ) deletion in the C-terminal II, they could be encoded by D genome.

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