A rapid, accurate, and sensitive densitometric TLC method has been established and validated for simultaneous analysis of eight β-lactam antibiotics (cephalexin, cefadroxil, and cefazolin (first-generation), cefaclor, cefuroxime, and cefuroxime axetil (second-generation), and ceftriaxone and cefotaxime (third-generation)). Chromatographic separation was achieved on silica gel F254 plates with chloroform-ethyl acetate-glacial acetic acid-water 4:4:4:1 (v/v) as mobile phase. Densitometric detection was performed at 275 nm. Statistical evaluation showed the performance of the method was satisfactory. Accuracy was in the range 98.30–100.85% and precision, as RSD, was from 0.4 to 2.45%.