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  • Author or Editor: Sarvesh Paliwal x
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The present study was aimed to assess the scientific appraisal of Phoenix sylvestris in the course of pharmacognostical properties and phytochemical parameters, as this has not been done yet. Pharmacognostic study mainly covered the macroscopic and microscopic features of the roots including powder microscopy and revealed the presence of pitted and spiral xylem vessels, lignified xylem fiber, and brown-colored stone cells. Physicochemical constants such as ash value, extractive value, and fluorescence analysis were assessed in the preliminary physicochemical screening. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, steroids, and phenolic compounds. The crude extract of the root was subjected to high-performance thin-layer chromatography (HPTLC) for the separation of components. HPTLC analysis was carried out on the methanolic extract of the root by using toluene–ethyl acetate–formic acid acid (5:4:1, v/v) as the mobile phase at 254-nm detection to quantify the amount of quercetin. The calibration range was 50–250 ng per band (r 2 = 0.994). The method was accurate in triplicate results at diff erent standard addition levels with average recovery of 99.40% for quercetin. Limits of detection and quantification were calculated as 20.94 ng per band and 43.90 ng per band, respectively. The quantity of quercetin in the methanolic extract was found to be as 3.13 mg g-1. The developed and validated HPTLC method can be used as a tool for standardization of roots in diff erent formulations using quercetin as the marker.

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A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination and validation of ursolic acid, β-sitosterol, lupeol, and quercetin in the methanolic fraction of Ichnocarpus frutescens L. was developed for the first time. For achieving good separation, a mobile phase of toluene‒ethyl acetate‒formic acid (8:2:0.1, v/v) was used. Densitometric determination was carried out at 500 nm for ursolic acid, 550 nm for β-sitosterol, 650 nm for lupeol, and 310 nm for quercetin in reflection–absorption mode, and the calibration curves were linear in the range of 100–600 ng per spot. During the analysis, the methanolic fraction of I. frutescens L. showed the presence of ursolic acid (0.34%), β-sitosterol (0.27%), lupeol (0.27%), and quercetin (0.26%). The proposed method is simple, precise, specific, and accurate. The obtained data can be used for routine analysis of reported biomarkers in crude drug and extracts. The simultaneous quantification and method validation of these biomarkers have not yet been reported in I. frutescens L., which may be utilized for the proper standardization of the plant.

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A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination of ursolic acid and β-sitosterol in the methanolic fraction of Paederia foetida L. leaves was developed for the first time. For achieving good separation, a mobile phase of toluene‒ethyl acetate‒formic acid (8:2:0.1, v/v) was used. The densitometric determination was carried out at 550 and 522 nm in reflection/absorption mode for ursolic acid and β-sitosterol. The calibration curves were linear in the range of 100-600 ng per spot for ursolic acid and β-sitosterol. During the analysis, the methanolic fraction of P. foetida L. leaves showed the presence of ursolic acid (0.12 ± 0.05%) and β-sitosterol (0.08 ± 0.12%). The proposed method is simple, precise, specific, accurate, less time-consuming, and cost-effective. The statistical analysis of the data obtained proves that the method is reproducible and selective and can be used for the routine analysis of the reported phenolic compounds in crude drug and extracts. The simultaneous quantification of these compounds has not yet been reported in P. foetida L. leaves which may be utilized for the proper standardization of the plant.

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