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  • Author or Editor: Shuge Tian x
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The contents of the four components in different growth stages of Artemisia rupestris L. was determined by thin-layer chromatographic scanning. Chloroform, methanol, formic acid, and water with the proportion of 6.35:0.63:0.17:0.07 were used as the developing solvent. The temperature was 28–32°C. The humidity was less than 30%. The scanning wavelengths were 250 nm and 352 nm. The linearity ranges of rupestonic acid, vitexicarpin, apigenin, and luteolin were 0.128–0.725, 0.0706–0.400, 0.0255–0.145, and 0.0172–0.0976 μg, respectively. The components had the corresponding correlation coefficients of 0.9909, 0.9908, 0.9971, and 0.9934, which showed a good linear relationship. Precision analyses showed a relative standard deviation of <5.0%. Stability studies showed that rupestonic acid, vitexicarpin, apigenin, and luteolin can be stable for at least 30 min at room temperature. Their average recovery percentages were 99.9%, 103.6%, 98.07%, and 99.9%, respectively. The contents of the four components in the different growth stages of A. rupestris L. were studied. The obvious changes of the four components could be used as the control for the quality of A. rupestris L. and the determination of the time of harvest.

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Summary

The aim of this work was to establish qualitative and quantitative methods for studying Guyinye residue extracts and Turkish gall (TG) cream. This study involved qualitative and quantitative analyses of gallic acid and methyl gallate and determined their preliminary antioxidant activity by high-performance thin-layer chromatography (HPTLC) and thin-layer chromatography-1,1-diphenyl-2-trinitrobenzene hydrazine (TLC-DPPH) in Guyinye residue extracts and TG cream. The thin-layer plate was a polyamide film and glacial acetic acid, methanol, ethyl acetate, and formic acid (10:6:2:1, volume ratio) were used as the developing agent. The scanning wavelength was 280 nm. Results showed that the RF values of gallic acid and methyl gallate were 0.57 ± 0.05 and 0.72 ± 0.05, respectively, and their linearity ranges were 0.001–0.005 and 0.00025–0.00125 mg with correlation coefficients of 0.9990 and 0.9994, respectively, which indicated a good linear relationship. The detection limits of gallic acid and methyl gallate were 3 and 75 ng, respectively, and their quantification limits were 10 and 250 ng, respectively. The average recovery was 98.59% and 98.33%, and the relative standard deviation (RSD) was 2.49% and 3.55%, respectively. Gallic acid was more remarkable than methyl gallate in antioxidant activity. Thus, HPTLC combined with TLC-DPPH, which can rapidly and accurately determine gallic acid and methyl gallate in Guyinye residue extracts and TG cream, is a simple, accurate, and rapid qualitative and quantitative method.

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A simple and rapid high-performance thin-layer chromatographic (HPTLC) method has been established for the analysis of rutin, rosmarinic acid, and glycyrrhizin, three active compounds from the decoction of Abnormal Savda Munziq (ASMq) prescription. The method employed silica gel GF254 thin-layer plates as the stationary phase, with ethyl acetate-formic acid-acetic acid-water (15:1:1:1.5, ½/½) as the mobile phase. Following development, plates were observed under ultraviolet (UV) light at 250 nm. The method was validated for linearity, precision, robustness, recovery, limit of detection (LOD), and limit of quantification (LOQ). Linear regression analysis data for the calibration plots showed good linear relationships (r) from 0.9992 to 0.9993. Recovery of the three compounds was between 97.86 and 100.77%. LOD and LOQ were in the range of 33.30–38.17 ng and 110.80–127.26 ng for the three compounds, respectively. The method is rapid, simple, effective, and easy-to-use for ASMq to perform routine quality control analysis and stability studies in commercial preparations.

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The aim of this study was to identify and quantitatively determine kaempferol, quercetin, and gallic acid in the methanol–water extract of the herb Euphorbia humifusa Willd. using high-performance thin-layer chromatography (HPTLC). The antioxidant compounds in E. humifusa Willd. were screened by thin-layer chromatography—1,1-diphenyl-2-trinitrobenzene hydrazine (TLC—DPPH). Kaempferol, quercetin, and gallic acid were used as the reference substances to optimize a highly suitable deployment system. Analysis was performed on silica gel G F254 plates with chloroform—ethyl acetate—formic acid with 5.2:4.4:1 proportion as the mobile phase. Antioxidant activity was screened preliminarily by spraying 10% DPPH ethanol solution on the developed silica gel plate. The quantification of E. humifusa Willd. was done at 300 nm for gallic acid and at 400 nm for quercetin and kaempferol. The linearity ranges of gallic acid, quercetin, and kaempferol were 0.168–0.448, 0.179–0.47, and 0.183–0.488 μg spot−1, respectively, with correlation coefficients of 0.9997, 0.9995, and 0.9995, respectively. Intra-day and inter-day precision studies showed a relative standard deviation (RSD) of <3.5%. Stability studies showed that gallic acid, quercetin, and kaempferol can be stable for at least 24 h at room temperature. The limit of detection (LOD) and limit of quantification (LOQ) were within the nanogram range for all three compounds. Average recovery was >96%. The developed method is simple and robust, with good stability and reproducibility. This method satisfies the requirements for quantitative determination and is effective in the quality control and evaluation of E. humifusa Willd.

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A simple, rapid, and effective high-performance thin-layer chromatographic method has been developed for the analysis and quantitative determination of ellagic acid, gallic acid, and methyl gallate in the galls of Quercus infectoria Olivier. Analysis was performed on silica gel G F254 TLC plates with toluene–ethyl acetate–formic acid (6:4.5:2, volume ratio) as the mobile phase. Densitometric detection was performed at 300 nm. The method was validated for linearity, precision, stability, recovery, and robustness. The limits of detection (LOD) and quantification (LOQ) were determined. The calibration curves showed a good linearity for ellagic acid, gallic acid, and methyl gallate with correlation coefficients of 0.9995, 0.9997, and 0.9996, respectively. The LOD and LOQ values were in the nanogram range for all three compounds. The average recovery was >96%. The amounts of ellagic acid, gallic acid, and methyl gallate in the galls of Q. infectoria were determined by using calibration graphs. The developed method is simple, with good precision, stability, recovery, and robustness. This method can satisfy the requirements for quantitative determination and provides a new and effective way for the quality monitoring and evaluation of galls of Q. infectoria.

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