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JPC - Journal of Planar Chromatography - Modern TLC
Authors: Selvadurai Muralidharan, Subramania Meyyanathan, Nithyanantham Muruganantham, Sekar Rajan, Kaliaperumal Krishnaraj, and Bhojraj Suresh

A simple, sensitive, precise, and rapid high-performance thin-layer chromatographic (HPTLC) method for analysis of dexibuprofen in pharmaceutical formulation has been developed and validated. The method uses aluminum foil HPTLC plates coated with silica gel 60F 254 as stationary phase and hexane-ethyl acetate-glacial acetic acid 7.5:2.5:0.5 ( v/v ) as mobile phase. Densitometric analysis of dexibuprofen and the internal standard (aceclofenac) was performed in reflectance mode at 217 nm. The system was found to give compact bands for dexibuprofen ( R F 0.50). Linear regression analysis of the calibration data revealed a good linear relationship between response and concentration in the range 50–300 ng per band ( r 2 = 0.9902). The method was validated for precision, accuracy, recovery, and robustness. The method has been successfully applied to analysis of an oral solid dosage formulation.

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JPC - Journal of Planar Chromatography - Modern TLC
Authors: Shrishailappa Badami, Mahesh Gupta, Noble Mathew, Subramania Meyyanathan, Bhojraj Suresh, and David Bendell

Among the complex mixture of biologically active compounds in the bark of Grewia tiliaefolia , a plant used in folklore, lupeol, a constituent of the bark, has been used as an analytical marker indicative of the quality of the plant. A sensitive and reliable quantitative high-performance thin-layer chromatographic method has been developed for the determination of lupeol from Grewia tiliaefolia . Chloroform extracts of bark from five different sources were used for HPTLC on silica gel with benzene-ethyl acetate, 95 + 5, as mobile phase. Under these conditions the R F of lupeol was 0.40. The calibration plot was linear in the range 0.5 to 1.5 μg lupeol and the correlation coefficient, 0.999, was indicative of good linear dependence of peak area on concentration. The mean assay of lupeol was 2.902 ± 0.243 mg g −1 bark. The method enables reliable quantification of lupeol and good resolution and separation of lupeol from other constituents of Grewia tiliaefolia . To ascertain the purity of the peak from the test sample its in-situ reflectance spectrum was compared with that from standard lupeol; clear superimposibility indicated the purity of the peaks. Recovery values from 98.00 to 99.78% showed the reliability and reproducibility of the method were excellent. The HPTLC method proposed for quantitative monitoring of lupeol in Grewia tiliaefolia is rapid, simple, and accurate and can be used for routine quality testing.

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