Authors:M. Takó, Elvira Farkas, Szabina Lung, Judit Krisch, Cs. Vágvölgyi, and T. Papp
Extracellular β-glucosidase activity of 94 strains, representing 24 species of the genera
Gilbertella, Mucor, Rhizomucor
was evaluated in submerged culture and under solid state fermentation on wheat bran.
G1 isolate showed the highest activity (70.9 U ml
) followed by other
(58.6–59.0 U ml
isolates (29.2–42.0 U ml
). Optimum temperature for enzyme production was 25 °C for
, and 30 °C for
strains. Enzymes of
strains proved to be thermotolerant preserving up to 92.8% residual activity after heating to 75 °C in the presence of cellobiose substrate. Enzymes of
chibinensis, R. miehei
strains were activated at acidic condition (pH 4). Glucose was a strong inhibitor for each fungal β-glucosidase tested but some of them showed ethanol tolerance up to 20% (v/v). Ethanol also activated the enzyme in these strains suggesting glycosyl transferase activity.