Authors:Szabolcs Varbiro, A. Biro, J. Cervenak, L. Cervenak, M. Singh, F. Banhidy, A. Sebestyen, G. Füst and Z. Prohászka
Anti-human Hsp60 autoantibodies — known risk factor of atherosclerosis — were investigated in a mouse model and in samples of healthy subjects: polyreactivity, presence in cord blood samples of healthy newborns and life-long stability were tested. In IgM hybridoma panel from mouse spleens, polyreactivity of anti-Hsp60 autoantibodies was studied. In healthy pregnant women, umbilical vein and maternal blood samples were collected after childbirth, anti-Hsp-60 and -65 IgM and IgG levels were measured. Life-long stability of anti-Hsp-60 levels was studied on healthy patients during 5 years. ELISA was used in all studies. Polyreactivity of IgM clones of newborn mice and lifelong stability of these autoantibodies in healthy adults were established. IgM anti-Hsp60 autoantibodies in cord blood of healthy human infants were present, however, there was no correlation between maternal and cord blood IgM anti-Hsp60 concentrations. It is proposed that presence of anti-Hsp60 autoantibodies — as part of the natural autoantibody repertoire — may be an inherited trait. Level of anti-Hsp60 autoantibodies may be an independent, innate risk factor of atherosclerosis for the adulthood.
Authors:M. Matrai, B. Szekacs, M. Mericli, G. Nadasy, M. Szekeres, F. Banhidy, G. Bekesi, E. Monos and Szabolcs Várbíró
Hypertension causes small vessel remodeling, vasomotor alterations. We investigated diameter, tone and mechanics of intramural small coronaries of female rats that received chronic angiotensin treatment to induce hypertension.Angiotensin II infusion (AII, 100 ng/bwkg/min, sc.) was used to establish hypertension in 10 female rats. Other 10 rats served as controls. Following 4 weeks of treatment, side branches of the left anterior descendant coronary (diameter∼200 μm) were isolated, cannulated and pressure-diameter curves were registered between 2–90 mmHg. Changes in vessel diameter were measured in Krebs solution, in the presence of thromboxane A2 receptor agonist (U46619, 10−6M), bradykinin (BK, 10−6M), and finally at complete relaxation (in Ca2+-free solution).Chronic AII treatment raised the mean arterial pressure (130±5 mmHg vs. 96±2 mmHg, average ±SEM) significantly. Wall thickness of the AII group was significantly greater (40.2±4.2 μm vs. 31.4±2.7 μm at 50 mmHg in Ca2+-free solution), but cross-section of the vessel wall did not differ. Tangentional wall stress and elastic modulus decreased significantly in hypertensive animals. Constrictions in the presence of U46619 were greater in the AII group (24.4± 5.6% vs. 14.5±3.3% at 50 mmHg).In hypertension, intramural small coronaries showed inward eutrophic remodeling, as a morphological adaptation following AII treatment enhanced thromboxane A2 — induced tone.
Authors:Levente Sára, GyL. Nádasy, P. Antal, M. Szekeres, A. Monori-Kiss, E. Horváth, A. Tőkés, G. Masszi, E. Monos and Szabolcs Várbíró
To clarify the effects of dihydrotestosterone (DHT)-induced polycystic ovary syndrome (PCOS) on arteriolar biomechanics in a rat model and the possible modulatory role of vitamin D3.Methods and Results The PCOS model was induced in female Wistar rats by ten-weeks DHT treatment. Arteriolar biomechanics was tested in arterioles by pressure arteriography in control as well as DHT- and DHT with vitamin D3-treated animals in contracted and passive conditions. Increased wall stress and distensibility as well as increased vascular lumen were detected after DHT treatment. Concomitant vitamin D3 treatment lowered the mechanical load of the arterioles and restored the vascular diameter.Conclusion The hyperandrogenic state resulted in more rigid, less flexible arteriolar walls with increased vascular lumen compared with controls. DHT treatment caused eutrophic remodelling of gracilis arteriole. These prehypertensive alterations caused by chronic DHT treatment were mostly reversed by concomitant vitamin D3 administration.
Authors:István Takács, Ilona Benkő, Erzsébet Toldy, Norbert Wikonkál, László Szekeres, Edit Bodolay, Emese Kiss, Zoltán Jambrik, Boglárka Szabó, Béla Merkely, Zsuzsa Valkusz, Tibor Kovács, András Szabó, Orsolya Grigoreff, Zsolt Nagy, Judit Demeter, Henrik Csaba Horváth, Nóra Bittner, Szabolcs Várbíró and Péter Lakatos
A D-vitamin anyagcseréje egyedülálló az emberi szervezetben. Hatása szerteágazó, szinte minden szervrendszerben érvényesül. Hiánya az egyik legnagyobb egészségügyi probléma a civilizált világban. A probléma megoldása széles körű összefogást sürget. Ezt felismerve, a D-vitamin-hiány következményeivel küzdő legnagyobb magyarországi orvostársaságok közös konszenzust dolgoztak ki a D-vitamin-hiány jelentőségéről, felismerési lehetőségeiről, a prevenció és a kezelés javasolt módjairól. A társaságok szakmai irányelvei mellett ennek a konszenzusnak az eredménye iránymutatást ad a gyakorló orvosoknak a D-vitamin-hiány megelőzéséhez és kezeléséhez. Emellett szeretné ráirányítani a szakmai döntéshozók és a laikus közönség figyelmét a probléma fontosságára.