Authors:P. Jankovics, T. Németh, J. Németh-palotás, and H. Kőszegi-szalai
A reversed-phase ion-pairing liquid chromatographic (RP-IP-HPLC) method for simultaneous assay of theophylline (TH), phenobarbital (PB), codeine (CD), and ephedrine (EP) in an extemporaneous (magistral) suppository was developed and validated and used to investigate a reported serious adverse event. Samples were dissolved in dichloromethane and extracted by two-step liquid-liquid extraction with acetate buffer (pH 5.0) and, subsequently, 0.1 m NaOH solution. Separation was performed on an end-capped C18 silica column with stepwise gradient elution. Sample preparation and chromatographic conditions were optimized on the basis of the pKa values of the analytes. Freedom from interference from the excipients, linearity (r2 > 0.999 for all the active ingredients), range (0.01–0.08 mg mL−1 for TH, CD, and EP; 0.0025–0.02 mg mL−1 for PB), intra-day and inter-day precision, and accuracy (recovery >95% for TH, CD, and EP and >90% for PB) of the method were demonstrated. Non-compliance of the examined product was confirmed.
Authors:Beáta Szabó, Á. Lakatos, T. Kőszegi, and L. Botz
We aimed to understand the effects of water stress on the alkaloid production in various developmental stages of poppy plants and the effect of stress on the alkaloids content in the capsules. Three stages of the life cycle of
L. were selected in our studies: Rosette, Flowering and Lancing developmental stages. Four types of water conditions were examined: Control, Withdrawal of Water, 50% Water Supply and Inundation.The morphological monitoring, results of Relative Water Content and proline content were used as indicators of stress. The result of the measurements in poppy leaves show that the secondary metabolites dramatically respond to these stress conditions. The constant water supply was beneficial for the accumulation of alkaloids in the capsules.