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  • Author or Editor: T. Sumíková x
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In 2013–2015 virulence in the wheat leaf rust population was evaluated on 17 Thatcher near-isogenic lines with leaf rust resistance genes. A total of 110 wheat leaf rust isolates were analyzed. Resistance genes Lr9 and Lr19 were effective to all tested isolates. Genes Lr24 and Lr28 conditioned resistance to 92% of the tested rust isolates. Thirty-seven winter wheat cultivars registered in Slovakia were analyzed for the presence of Lr10, Lr24, Lr26, Lr34 and Lr37 using tightly linked molecular markers. Gene Lr37 was the most common in the tested cultivars. Leaf rust resistance was also tested in 13 wheat cultivars at the seedling stage with representative pathotypes of leaf rust.

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Amplified fragment length polymorphism (AFLP) analysis was applied to genotype 186 Czech isolates of the Fusarium species responsible for head blight disease in wheat. Using 15 primer combinations, 694 polymorphic fragments were detected. The effective number of patterns was dependent on both the identity of the selective primer combination and on which Fusarium species was involved. The most informative primer combinations were CAT + AT, CAT + AC and CTA + AG, and a triplex assay based on CAT was able to distinguish all 186 isolates from each other. A principal component analysis identified six phylogenetic clusters, and this genetic architecture was supported by an analysis of population structure. AFLP profiling is an appropriate method for uncovering differences between these groups of Fusarium species.

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