Authors:L.-M. Zhang, W.-G. Xie, T.-T. Wen, and X. Zhao
The thermal behavior of five free anthraquinones (chrysophanol, emodin, physcion, aloe-emodin, and rhein) from rhubarb had
been investigated using TG, DTG and DTA technique. The results show that all the free anthraquinones have the similar TG and
DTG curve shapes, however, due to the substituted groups attached on the skeleton of 1,8-dihydroxy anthraquinone are different,
every anthraquinone has different mass loss features. Moreover, all the DTA curves of these free anthraquinones have two obviously
characteristic peaks, but with special curvilinear types, peak location and peak values. Therefore, thermal analysis (TA)
characteristics of anthraquinones above mentioned could be established, and it is possible to easily distinguish these anthraquinones
by using TA technique.
Authors:J. Li, W. Dai, G. Xiao, H. Wang, Z. Zhang, and T. Wu
99Tc is an important radionuclides related to repository safety assessment. The mobility pertechnetate (TcO4−) can be reduced to immobility technetium(IV) hydrous oxides (TcO2·nH2O) by Fe(II)-bearing minerals. In China, Gaomiaozi (GMZ) bentonite is regarded as the favorable candidate backfilling material
for the HLW repository, which is contained some FeO. The diffusion behavior of 99Tc was investigated in GMZ bentonite by through- and out-diffusion methods. The effective diffusion coefficient (De), the accessible porosity (εacc), apparent diffusion coefficient (Da) and distribution coefficient (Kd) were decreased with the increasing of dry density. The De values were (2.8 ± 0.2) × 10−11 m2/s and (3.5 ± 0.2) × 10−12 m2/s at dry density of 1,600 and 1,800 kg/m3, respectively. It was indicated that the dominating species was TcO4− during the diffusion processing. While, out-diffusion results showed that part of TcO4− may be reduced by Fe(II). The relationship of De and εacc could be described by Archie’s law with exponent n = 2.4 for 99Tc diffusion in GMZ bentonite. Furthermore, the relationship between Da and dry density (ρ) was exponential.
Authors:F. Z. Yin, W. Yin, X. Zhang, T. L. Lu, and B. C. Cai
The ripe fruits of Schisandrae chinensis have a long history of use in traditional Chinese medicine to treat diseases and improve health. There is substantial evidence that lignan constituents are mainly responsible for the beneficial effects of this plant medicine. The amounts of the major bioactive lignans in this plant vary widely with species, habitat, and the collecting time, and as such, establishment of an HPLC fingerprint for quality control of this herbal medicine is of particular importance. To achieve this, ten batches of Fructus schisandrae chinensis were collected from Tieli, in China, and their chemical components were analyzed under optimized HPLC conditions. On the basis of the chromatographic data, a consistent HPLC fingerprint pattern containing 20 common peaks was obtained. Among these common peaks, six were identified as schizandrin, schizandrol B, schisantherin, deoxyschiandrin, γ-schizandrin B, and schizandrin C. On the basis of this HPLC fingerprint and principal-components analysis, the quality of fifteen samples from different producing areas of China was objectively assessed, and the species difference between Fructus schisandrae sphenantherae and Fructus schisandrae chinensis was clearly differentiated. To summarize, the data described in this study offer valuable information for quality control and proper use of Fructus schisandrae chinensis.
Authors:L.J. Wu, Y. Shang, T. Liu, W.J. Chen, B.L. Liu, L.Q. Zhang, D.C. Liu, B. Zhang, and H.G. Zhang
In this study, the cDNA of homocysteine S-methyltransferase was isolated from Aegilops tauschii Coss., with the gene accordingly designated as AetHMT1. Similar to other methyltransferases, AetHMT1 contains a GGCCR consensus sequence for a possible zinc-binding motif near the C-terminal and a conserved cysteine residue upstream of the zinc-binding motif. Analysis of AetHMT1 uncovered no obvious chloroplast or mitochondrial targeting sequences. We functionally expressed AetHMT1 in Escherichia coli and confirmed its biological activity, as evidenced by a positive HMT enzyme activity of 164.516 ± 17.378 nmol min−1 mg−1 protein when catalyzing the transformation of L-homocysteine. Compared with the bacterium containing the empty vector, E. coli harboring the recombinant AetHMT1 plasmid showed much higher tolerance to selenate and selenite. AetHMT1 transcript amounts in different organs were increased by Na2SeO4 treatment, with roots accumulating higher amounts than stems, old leaves and new leaves. We have therefore successfully isolated HMT1 from Ae. tauschii and characterized the biochemical and physiological functions of the corresponding protein.
Authors:W.M. Du, F.H. Wang, H.Y. Zhang, B.Z. Jiang, X.Y. Chen, W. Zhang, Y. Xie, and Z.T. Sheng
Present research on prebiotics focuses on either polysaccharides or polyphenols. This study compared the individual and combined impact of polysaccharide, quercetin, and gallic acid (GA) treatment on three human faecal strains. In vitro pure culturing and correlation analysis confirmed that the growth of both beneficial microbe B. longum subsp. longum (0.695, 0.205: R2, slope, respectively) and pathogenic C. perfringens (0.712, 0.085: R2, slope, respectively) increased due to polysaccharide treatment, and only GA treatment would inhibit C. perfringens (0.789, –0.165: R2, slope, respectively) growth. In vivo studies also revealed that genome copies of Bifidobacterium increased and C. perfringens decreased in the faeces, when a blend of the three nutrients rather than single polysaccharide or polyphenols were fed to rats. These data suggested that combined prebiotic treatment improved human faecal strain composition better than single treatment.
Authors:J.Q. Xu, L. Wang, B.L. Liu, T.F. Xia, D.C. Liu, X. Chang, T.W. Zhang, H.G. Zhang, and Y.H. Shen
As one of the world’s earliest domesticated crops, barley is a model species for the study of evolution and domestication. Domestication is an evolutionary process whereby a population adapts, through selection; to new environments created by human cultivation. We describe the genome-scanning of molecular diversity to assess the evolution of barley in the Tibetan Plateau. We used 667 Diversity Arrays Technology (DArT) markers to genotype 185 barley landraces and wild barley accessions from the Tibetan Plateau. Genetic diversity in wild barley was greater than in landraces at both genome and chromosome levels, except for chromosome 3H. Landraces and wild barley accessions were clearly differentiated genetically, but a limited degree of introgression was still evident. Significant differences in diversity between barley subspecies at the chromosome level were observed for genes known to be related to physiological and phenotypical traits, disease resistance, abiotic stress tolerance, malting quality and agronomic traits. Selection on the genome of six-rowed naked barley has shown clear multiple targets related to both its specific end-use and the extreme environment in Tibet. Our data provide a platform to identify the genes and genetic mechanisms that underlie phenotypic changes, and provide lists of candidate domestication genes for modified breeding strategies.
Authors:P. Aarnio, J. Ala-Heikkilä, A. Isolankila, A. Kuusi, M. Moring, M. Nikkinen, T. Siiskonen, H. Toivonen, K. Ungar, and W. Zhang
Linssi is a Structured Query Language (SQL) database for HPGe gamma-ray spectrometry. It covers the whole production chain from
sample preparation to final analysis results. Static or mobile sampling and measurement and multiple sample types are supported.
In addition, each sample can be split or combined any number of times. A sample may be measured multiple times and each measurement
multiply analyzed as well. With Linssi, measurement setups with detectors, shields, attenuators and source geometries can be defined. Full control of calibrations,
their histories and tracing of each calibration point back to its corresponding analysis and calibration measurement is supported.
Linssi database and user scripts are available from http://linssi.hut.fi/radphys/linssi.
Authors:K. Ungar, W. Zhang, P. Aarnio, J. Ala-Heikkila, H. Toivonen, T. Siiskonen, A. Isolankila, A. Kuusi, M. Moring, and M. Nikkinen
Linux System for Spectral Information (LINSSI1) is a SQL database and established under Linux. Currently it is compatible with HPGe gammaspectra analysis software UniSampo,
Shaman and Aatami. Based on this database and software, an automated analysis pipeline has been setup for Canadian CTBT radiological
monitoring networks. This paper has investigated the performance of this pipeline in its capabilities and reliabilities of
rapid small peak search, nuclide identification, and radionuclide activity concentration evaluation. Up to now, more than
80 thousand daily monitoring gamma-spectra have been automatically received and processed, the results have been stored in
database. The pipeline nuclide detection limits is satisfied for environmental radiation monitoring and nuclear emergency