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  • Author or Editor: Usmangani Chhalotiya x
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A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of torsemide and amiloride hydrochloride in the pharmaceutical dosage form. Thin-layer chromatographic (TLC) aluminum plates precoated with silica gel 60 F254 were used as the stationary phase, while chloroform-methanol-ammonia (7.5: 3.5: 1, v/v) as mobile phase was used. The R F values observed were 0.46 ± 0.01 and 0.24 ± 0.01 for torsemide and amiloride hydrochloride, respectively. The densitometric analysis was carried out in absorbance mode at 286 nm. The method was linear in the range of 100–600 ng spot−1 for torsemide and 50–300 ng spot−1 for amiloride hydrochloride. The method was validated as per the International Conference on Harmonization (ICH) guidelines. The limit of detection and limit of quantitation were found to be 19.94 ng spot−1 and 100 ng spot−1, respectively, for torsemide. The limit of detection and limit of quantitation were found to be 12.86 ng spot−1 and 50 ng spot−1, respectively, for amiloride. The proposed method was successfully applied to the estimation of torsemide and amiloride hydrochloride in the pharmaceutical dosage form.

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A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of paracetamol, dicyclomine hydrochloride, and mefenamic acid in combined dosage form. The method employed high-performance thin-layer chromatography (HPTLC) aluminum plates precoated with silica gel 60 F254 as the stationary phase, while the solvent system was toluene-ethyl acetate-methanol (6:3:1, v/v). The R F values were observed to be 0.27 ± 0.02, 0.41 ± 0.03, and 0.61 ± 0.02 for paracetamol, dicyclomine hydrochloride, and mefenamic acid, respectively. The separated spots were densitometrically analyzed in absorbance mode at 290 nm. The method was linear in the range of 300-1800 ng band−1 for paracetamol, 400–2400 ng band−1 for dicyclomine hydrochloride, and of 150–1500 ng band−1 for mefenamic acid. The method was validated with respect to accuracy, precision, specificity, sensitivity, and robustness. The limits of detection for paracetamol, dicyclomine hydrochloride, and mefenamic acid were found to be 35, 109, and 42 ng band−1, respectively. The limits of quantification for paracetamol, dicyclomine and mefenamic acid were found to be 116, 360, and 140 ng band−1, respectively. The method was successfully applied to the estimation of all three drugs in combined tablet dosage form.

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A sensitive and precise high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous estimation of clozapine and aripiprazole in combination. The method employed HPTLC aluminum plates pre-coated with silica gel 60 F254 as the stationary phase, while the solvent system was toluene‒methanol‒ethyl acetate‒ammonia (6.5:2.5:1:0.1, v/v). The R F values were observed to be 0.43 and 0.60 for clozapine and aripiprazole, respectively. Densitometric analysis was carried out in absorbance mode at 218 nm. The method was linear in the range of 200–1600 ng per band for clozapine and 100-800 ng per band for aripiprazole. The stress degradation study was performed, and it was found that clozapine was susceptible to acid hydrolysis, base hydrolysis, and photolytic degradation study. Aripiprazole was susceptible to oxidative stress degradation study. The method was validated and applied successfully for the estimation of both drugs in the synthetic mixture.

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