Authors:S. Grace, V. Meera, S. Balakrishnan and H. Sarma
Three different radiotracers of testosterone incorporating125I at position 3 or 19 were prepared and tested for usability in radioimmunoassays employing the antisera raised at our laboratory. Histamine was radioiodinated and conjugated to suitable testosterone derivatives. On optimization, tracers with high purity, good immunoreactivity and long stability could be obtained for all the three tracers. However, the usability of a tracer will also depend on the antiserum used and this was also demonstrated using different pairs of antisera and tracers.