Search Results

You are looking at 1 - 3 of 3 items for

  • Author or Editor: Vanka Sarma x
  • Refine by Access: All Content x
Clear All Modify Search

A simple, accurate, and rapid high-performance thin-layer chromatographic (HPTLC) method has been established and validated for the simultaneous quantification of the four biologically active dibenzyl cyclooctadiene lignans, i.e., schisandrin (1), gomisin B (2), deoxyschisandrin (3), and gomisin N (4) from the hexane extract of Schisandra grandiflora (Wall.) Hook. f. & Thoms. Separation was performed on silica gel 60 F254 plates using a saturated mixture of toluene-ethyl acetate-methanol (6:1:1 v/v) as the mobile phase. Quantitation was performed using densitometric absorption-reflection mode at 225 nm. The method was validated for its selectivity, linearity, precision, and accuracy. HPTLC was hyphenated with a mass spectrometer as an additional tool for the confirmation of the markers using an interface. The developed method is simple and convenient which can be applied for the regular quality analysis of the raw plant material used in Chinese traditional herbal formulations.

Restricted access

Plants of the Hedychium genus are perennial rhizomatus plants belonging to the Zingiberaceae family. Extracts of Zingiberacaea have long been used in traditional medicine. A simple, precise, and convenient HPTLC method has been established for analysis of hedychenone, the major marker compound extracted from the rhizomes of Hedychium spicatum (Buch-Hem). Chromatography was performed on silica gel 60F 254 plates with ethyl acetate-hexane, 20 + 80 ( v/v ), as mobile phase. Detection and quantification were performed densitometrically at λ max = 254 nm with hedychenone as external standard. The method is characterized by high sensitivity and linearity over a wide range of concentrations.

Restricted access

The isolation and characterization of bioactive compounds from medicinal plants is usually a significant challenge in phytochemical analysis because of the natural chemical complexity of plant extracts. However, there exists a need for analytical tools which can quantitatively separate and characterize the components from these biosources with greater chromatographic selectivity and lesser analytical run times that facilitate the evaluation with enhanced separation profiles. Hyphenation of thin-layer chromatography (TLC/HPTLC) with mass spectrometry (MS) is an alternative for screening herbal extracts because of its rapid analysis and ability to aid structural characterization with powerful analytical capacity. The aim of the present study was to develop a sophisticated analytical method which utilizes HPTLC–MS coupling for the chromatographic profiling and evaluation of the therapeutically important genus Piper (Piperaceae). In this study, six marker compounds, namely, trichostachine, piperine, 4,5-dihydropiperlonguminine, guineensine, pellitorine, and sesamin were analyzed and quantified in extracts of Piper nigrum L. and compared with those of Piper longum L. and Piper chaba Hunter. All the samples tested showed similar phytochemical profiles, but the contents of the active ingredients varied. Additionally, HPTLC–MS further allowed confirming the identification of the constituents in the analyzed samples with greater chromatographic selectivity where HPTLC facilitated a selective chromatographic resolution, while MS offered an efficient characterization of the target compounds in one analytical run. The study finds a potential utility in adopting HPTLC–MS as a rapid and high throughput method for the efficient quantification and identification of marker compounds from medicinal plants.

Restricted access