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  • Author or Editor: Vidya Dighe x
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Eugenol is used as a flavor in the food industry, has a variety of biological activity, and can serve as a biomarker. Because eugenol is present in the leaves of Cinnamomum tamala Nees and Eberm., which are used as a spice, a sensitive and reliable quantitative high-performance thin-layer chromatographic method has been established for quantification of the compound in the leaves of the plant. A methanol extract of the powder of dried leaves of Cinnamomum tamala Nees and Eberm. was applied to silica gel 60 F 254 TLC plates and these were developed with toluene-ethyl acetate-formic acid, 90 + 10 + 01 ( v/v ), as mobile phase. Detection and quantitation was performed by densitometry at λ = 280 nm. The accuracy of the method was checked by conducting recovery studies for two different levels of eugenol; the average recovery was found to be 98.39%. The average eugenol content, as estimated by use of the proposed method, was 5.405 mg g −1 . The HPTLC method proposed for the quantitative monitoring of eugenol in Cinnamomum tamala leaf powder is rapid, simple, and precise.

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A sensitive high-performance thin-layer chromatographic method has been established for quantification of apigenin in dried root powder from Gmelina arborea Linn. Chromatographic separation was performed on silica gel plates with chloroform-acetone-formic acid, 7.6:1.6:0.8 ( v / v ), as mobile phase. The plates were scanned densitometrically at 340 nm. The method was validated for precision and accuracy. The relative standard deviation for instrumental precision, intra-assay precision, and intermediate precision was <2%. Percentage recovery was 98.87.

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A sensitive and reliable high-performance thin layer chromatographic method has been developed for quantitation of camptothecin in the dry stem powder of Nothapodytes foetida (Wight) Sleumer. A methanolic extract of the dry powder was chromatographed on silica gel 60F 254 plates with toluene-acetonitrile-glacial acetic acid, 6.5 + 3.5 + 0.1 ( v / v ), as mobile phase. Detection and quantitation were performed by densitometric scanning, in fluorescence mode at λ = 370 nm, by use of a mercury lamp. The accuracy of the method was checked by determination of recovery, using the standard-addition method. Recovery was 99.49%. The average camptothecin content of the powder was 0.059%. The method is rapid, simple, and precise.

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A normal-phase high-performance thin-layer chromatographic (HPTLC) method has been established for quantitative estimation of itopride hydrochloride, a gastroprokinetic agent, in its pharmaceutical formulation and in the bulk drug substance. Analysis was performed on silica gel 60F 254 TLC plates with methanol-ethyl acetate-toluene-triethylamine, 1.0 + 2.5 + 6.0 + 0.5 ( v/v ), as mobile phase. Detection and quantitation were performed densitometrically at 1 = 230 nm. Diltiazem hydrochloride was used as the internal standard. Response to itopride hydrochloride was found to be linear in the concentration range 50.00 to 2000.00 μg mL −1 . The method was validated for accuracy and precision. Accuracy was checked by conducting recovery studies; the average recovery was found to be 99.27%. The method is simple, rapid, and precise and can be used for routine quality control.

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A sensitive and accurate high-performance thin-layer chromatographic method has been developed, validated, and used for quantification of dopamine in dried whole-plant powder of Portulaca oleracea Linn. The powder was extracted with 0.1 m HCl and the extract was separated on aluminum HPTLC plates coated with silica gel 60 F 254 , with n -butanol-glacial acetic acid-distilled water 7.0:2.0:1.0 ( v/v ) as mobile phase. Detection and quantitation were performed by densitometry, with a deuterium lamp, at 280 nm. The response to dopamine reference standard was linear in the concentration range 45.0 to 110.0 μg cm −3 . The validated method was used for quantitative analysis of dopamine in Portulaca oleracea Linn and can be used for routine quality-control analysis of whole-plant powder of Portulaca oleracea Linn.

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A normal-phase high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous quantitative determination of diclofenac sodium and paracetamol in a pharmaceutical formulation and in bulk drug powder. The analysis was performed on silica gel 60F 254 HPTLC plates with toluene-ethyl acetate-methanol-formic acid, 5.0 + 4.0 + 1.0 + 0.01 ( v / v ), as mobile phase. Detection and quantitation were performed densitometrically at λ = 260 nm. Aceclofenac was used as the internal standard. Responses of diclofenac sodium standard and paracetamol standard were linear functions of concentration in the ranges 30–800 ng μL −1 and from 300–2000 ng μL −1 , respectively. The method was validated to determine its accuracy and precision. Accuracy was checked by conducting recovery studies; average recovery from the pharmaceutical preparation was 99.57% for diclofenac sodium and 100.51% for paracetamol. This HPTLC method developed for simultaneous quantitative determination of diclofenac sodium and paracetamol in pharmaceutical preparations is simple, rapid, and precise and can be used for routine quality control. The method was also used to determine the amounts of diclofenac sodium and paracetamol in a mixture of their respective bulk drug powders.

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