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  • Author or Editor: Vijay Gokarn x
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A sensitive high-performance thin-layer chromatographic method has been established for quantification of apigenin in dried root powder from Gmelina arborea Linn. Chromatographic separation was performed on silica gel plates with chloroform-acetone-formic acid, 7.6:1.6:0.8 ( v / v ), as mobile phase. The plates were scanned densitometrically at 340 nm. The method was validated for precision and accuracy. The relative standard deviation for instrumental precision, intra-assay precision, and intermediate precision was <2%. Percentage recovery was 98.87.

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A sensitive and reliable high-performance thin layer chromatographic method has been developed for quantitation of camptothecin in the dry stem powder of Nothapodytes foetida (Wight) Sleumer. A methanolic extract of the dry powder was chromatographed on silica gel 60F 254 plates with toluene-acetonitrile-glacial acetic acid, 6.5 + 3.5 + 0.1 ( v / v ), as mobile phase. Detection and quantitation were performed by densitometric scanning, in fluorescence mode at λ = 370 nm, by use of a mercury lamp. The accuracy of the method was checked by determination of recovery, using the standard-addition method. Recovery was 99.49%. The average camptothecin content of the powder was 0.059%. The method is rapid, simple, and precise.

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A normal-phase high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous quantitative determination of diclofenac sodium and paracetamol in a pharmaceutical formulation and in bulk drug powder. The analysis was performed on silica gel 60F 254 HPTLC plates with toluene-ethyl acetate-methanol-formic acid, 5.0 + 4.0 + 1.0 + 0.01 ( v / v ), as mobile phase. Detection and quantitation were performed densitometrically at λ = 260 nm. Aceclofenac was used as the internal standard. Responses of diclofenac sodium standard and paracetamol standard were linear functions of concentration in the ranges 30–800 ng μL −1 and from 300–2000 ng μL −1 , respectively. The method was validated to determine its accuracy and precision. Accuracy was checked by conducting recovery studies; average recovery from the pharmaceutical preparation was 99.57% for diclofenac sodium and 100.51% for paracetamol. This HPTLC method developed for simultaneous quantitative determination of diclofenac sodium and paracetamol in pharmaceutical preparations is simple, rapid, and precise and can be used for routine quality control. The method was also used to determine the amounts of diclofenac sodium and paracetamol in a mixture of their respective bulk drug powders.

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