This study aimed to investigate the effects of single local vibration (LV) with and without blood flow restriction (BFR) on muscle activity and hormonal responses. A total of 12 physically inactive males were exposed to 10 sets of intermittent LV (35–40 Hz) on unilateral mid-quadriceps in the supine lying position and LV + BFR (inflated to 140 mmHg) sessions in a repeated-measures randomized crossover design, with a 1-week interval separating the sessions. The results indicated that the electromyography values from the rectus femoris during LV + BFR were greater than those during LV (p < 0.05). LV + BFR caused a minor increase in the lactate (LA) response (p < 0.05); LV with or without BFR failed to elicit change in growth hormone (GH) and testosterone (T) levels (p > 0.05). Cortisol (C) levels were decreased postexercise in both the sessions (p < 0.05). In conclusion, BFR elicited higher increase in muscle activity and metabolic response, but it did not induce hormonal responses. The exposure of LV and LV + BFR may only have a relief effect as detected by the reduction in C levels, probably because the LV did not elicit sufficient stimulus to the muscles.
Cyclic neutron activation analysis method was conducted for determination of Se in food samples. High accuracy and good precision
were proved by analyzing certified reference materials (CRMs) of chicken (GBW10018), rice (GBW10010) and cabbage (GBW10014).
The detection limits for the three CRMs reached 0.16, 0.66 and 1.2 ng after 6 cycles at the 161.9 keV γ-peak from 77mSe, under a neutron flux of 9.0 × 1011 n cm−2 s−1 and the conditions of 30 s irradiation, 2 s decay, 30 s counting and 2 s waiting, significantly lower than those of conventional
neutron activation analysis without any cycles, which were 0.94, 3.6 and 4.3 ng, respectively.
Authors:S. Tang, L. Li, Q.Y. Zhou, W.Z. Liu, H.X. Zhang, W. Z. Chen, and Y.F. Ding
Gibberellins (GAs) are a class of plant hormones that play important roles in diverse aspects during plant growth and development. A series of GA synthesis and metabolism genes have been reported or proved to have essential functions in different plant species, while a small number of GA 2-oxidase genes have been cloned or reported in wheat. Previous studies have provided some important findings on the process of GA biosynthesis and the enzymes involved in its related pathways. These may facilitate understanding of the complicated process underlying GA synthesis and metabolism in wheat. In this study, GA 2-oxidase genes TaGA2ox1-1, TaGA2ox1-2, TaGA2ox1-3, TaGA2ox1-4, TaGA2ox1-5, and TaGA2ox1-6 were identified and further overexpressed in rice plants to investigate their functions in GA biosynthesis and signaling pathway. Results showed overexpression of GA 2-oxidase genes in rice disrupted the GA metabolic pathways and induced catalytic responses and regulated other GA biosynthesis and signaling pathway genes, which further leading to GA signaling disorders and diversity in phenotypic changes in rice plants.
Authors:W. Li, Z.Y. Chen, Z. Li, X.F. Zhao, Z.E. Pu, G.Y. Chen, Q.T. Jiang, Y.M. Wei, and Y.L. Zheng
To study the development of starch granules in polyploid wheats, we investigated the expression of starch synthetic genes between the synthetic hexaploid wheat SHW-L1, its parents T. turgidum AS2255 and diploid Ae. tauschii AS60. The synthetic hexaploid wheat SHW-L1 showed significantly higher starch content and grain weight than its parents. Scanning electron microscopy (SEM) showed that SHW-L1 rapidly developed starch granules than AS2255 and AS60. The amount of B-type granule in AS60 was less than that in SHW-L1 and AS2255. RT-qPCR result showed that the starch synthetic genes AGPLSU1, AGPLSU2, AGPSSU1, AGPSSU2, GBSSI, SSIII, PHO1 and PHO2 expressed at earlier stages with larger quantity in SHW-L1 than in its parents during wheat grain development. The expression of the above mentioned genes in AS60 was slower than in SHW-L1 and AS2255. The expression pattern of starch synthase genes was also associated with the grain weight and starch content in all three genotypes. The results suggested that the synthetic hexaploid wheat inherited the pattern of starch granule development and starch synthase gene expression from tetraploid parent. The results suggest that tetraploid wheat could plays more important role for starch quality improvement in hexaploid wheat.
Authors:G. D. Ye, H. Zhou, J. W. Yang, Z. H. Zeng, and Y. L. Chen
Photoinitiating behaviors of oligo(α-aminoketones) (OAK) macrophotoinitiator
containing aminoalkylphenone group on free-radical photopolymerization had
been investigated by differential photo-calorimetry (DPC). The macrophotoinitiator
showed comparative performance with those commercial photoinitiators with
lower molecular mass. The effect of photoinitiator concentrations and UV intensity
on the polymerization rate was investigated, and the value of exponential
factor was found to be 0.5 at the beginning of polymerization, suggesting
that the photopolymerization initiated by OAK followed biradical termination
mechanism. Photosensitizer isopropyl thioxanthone (ITX) and oxygen severely
restricted the polymerization in these systems. Photoinitiators with lower
molecular mass showed higher reactivity than those with higher molecular mass.
Authors:B. Chen, L.-W. Li, Y.-J. Lin, Z.-H. Wang, and G.-D. Lu
Rice sheath blight, caused by Rhizoctonia solani, is the most serious disease in the southern rice producing regions of China. The use of resistant varieties is the most economic strategy to control the disease. In this paper, a seedling inoculation method was used to evaluate rice germplasm resources for resistance to sheath blight. A total of 363 rice varieties were evaluated with a set of R. solani isolates. The results indicated that the rice varieties generally lacked resistance to R. solani, and no highly resistant/immune (HR) variety was found. However, two varieties displayed clear resistance (R) and 37 showed moderate resistance (MR) to the fungus. Overall, hybrid rice varieties have better resistance than conventional rice varieties, and among hybrid rice varieties, those with the II-32A sterile line genetic background were the most resistant. The results also indicated significant interactions between rice varieties and pathogen isolates, suggesting that an understanding of local R. solani populations is needed when recommending varieties to local growers.
Authors:H.-L. Liu, S.-J. Liu, Z.-L. Xiao, Q.-Y. Chen, and D.-W. Yang
Excess molar enthalpies of binary mixtures for tributyl
phosphate (TBP)+methanol/ethanol were measured with a TAM air Isothermal calorimeter
at 298.15 K and ambient. The results for xTBP+(1–x)CH3OH
are negative in the whole range of composition, while the values for xTBP+(1–x)C2H5OH
change from positive values at low x to
small negative values at high x. The experimental
results have been correlated with the Redlich–Kister polynomial. IR
spectra of the mixtures were measured to investigate the effect of hydrogen
bonding in the mixture.
Authors:Q. Chen, M. Cao, W. Xiang, Q. Sun, J. Zhang, R. Hou, Z. Yan, Z. Yang, J. Liu, and J. Zhao
The forward and reverse cDNA subtractive libraries before and after the toxic effect of α-amanitin were constructed by suppression subtractive hybridization and randomly selected clones from each subtractive library were screened by PCR and dot blot hybridization. A total of 85 genes with altered expression were finally identified, with 41 genes from the forward library and 44 from the reverse library. Subsequently, the antagonistic effects of candidate traditional Chinese medicines were evaluated based on the genetic transcription levels of the genes with significant altered expression, including Catnβ, Flt3-L, IL-7r and Rpo2-4. The results indicated that
(L.) Gaert and
had significant down-regulated effects on the transcription level of Catnβ that was up-regulated by α-amanitin, and the two herbs also up-regulated the transcription levels of Flt3-L and Rpo2-4.
(L.) had significant up-regulated effects on the IL-7r that was down-regulated by α-amanitin. These preliminary studies suggested that
were effective antagonists against the toxicity of α-amanitin.
Authors:W.M. Du, F.H. Wang, H.Y. Zhang, B.Z. Jiang, X.Y. Chen, W. Zhang, Y. Xie, and Z.T. Sheng
Present research on prebiotics focuses on either polysaccharides or polyphenols. This study compared the individual and combined impact of polysaccharide, quercetin, and gallic acid (GA) treatment on three human faecal strains. In vitro pure culturing and correlation analysis confirmed that the growth of both beneficial microbe B. longum subsp. longum (0.695, 0.205: R2, slope, respectively) and pathogenic C. perfringens (0.712, 0.085: R2, slope, respectively) increased due to polysaccharide treatment, and only GA treatment would inhibit C. perfringens (0.789, –0.165: R2, slope, respectively) growth. In vivo studies also revealed that genome copies of Bifidobacterium increased and C. perfringens decreased in the faeces, when a blend of the three nutrients rather than single polysaccharide or polyphenols were fed to rats. These data suggested that combined prebiotic treatment improved human faecal strain composition better than single treatment.