Authors:G.C. Zhou, H.C. Shi, X.J. Yu, J.C. Yuan, Q. Guo, C.Y. Zhao, Q. Sun and Y.P. Ke
Male sterile mutants play an important role in the utilisation of crop heterosis. Male sterile plants were found in S5 generations of maize hybrid ZH2, through continuous sib-mating by using the fertile plants in the same population, we obtained a male sterile sibling population K932MS including sterile plants K932S and a fertile plant K932F. The objective of this study was to clarify the genetic characterisation and abortion characteristics by nucleus and cytoplasm effect analyses, cytoplasm grouping, and cytological observation. The results showed that no difference was found between K932S and K932F in the vegetative growth stage, but K932S had no emerging anther or pollen grains. The segregation ratio of fertile plants to sterile plants was 1:1 in the sibling progenies, while it was 3:1 in self-crossing progenies of K932F. The sterility of K932S could be restored among reciprocal progenies when seven normal inbred lines were used as females respectively. The fertility expression of K932S crossed with 30 testers would be changed in different test-crosses and some backcross progenies. The C-type restorer Zifeng-1 (Rf4Rf4) was able to restore the fertility of K932S, and the specific PCR amplification bands of K932MS were consistent with CMSCMo17. The anther of K932S began abortion at dyad with its tapetum expanded radically and vacuolated: this induced abnormality in the shapes of both dyads and tetrads. The microspore could not develop normally, and then it collapsed and gradually disappeared. Hence, K932MS is a C-type cytoplasmic male sterile mutant with a pollen-free, stable inheritance: it has potential application value for further research.
Authors:Y. C. Xiao, L. T. Liu, J. J. Bian, C. Q. Yan, L. Ye, M. X. Zhao, Q. S. Huang, W. Wang, K. Liang, Z. F. Shi and X. Ke
Shuganjieyu (SGJY) capsule is a classical formula widely used in Chinese clinical application. In this paper, an ultra-performance liquid chromatography coupled with electrospray ionization and ion trap mass spectrometry has been established to separate and identify the chemical constituents of SGJY and the multiple constituents of SGJY in rats. The chromatographic separation was performed on a C18 RRHD column (150 × 2.1 mm, 1.8 μm), while 0.1% formic acid–water and 0.1% formic acid–acetonitrile was used as mobile phase. Mass spectral data were acquired in both positive and negative modes. On the basis of the characteristic retention time (Rt) and mass spectral data with those of reference standards and relevant references, 73 constituents from the SGJY and 15 ingredients including 10 original constituents and 5 metabolites from the rat plasma after oral administration of SGJY were identified or tentatively characterized. This study provided helpful chemical information for further pharmacology and active mechanism research on SGJY.