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  • Author or Editor: Xiaoping Wu x
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Abstract

Purpose

This study aimed to evaluate and identify the value and explore the mechanisms of Angiogenic Factor with G-patch and FHA domains 1 (AGGF1) in postoperative cognitive dysfunction (POCD).

Methods

Rats were separated into four different groups, namely sham, isoflurane, isoflurane + recombinant human Aggf1 (rh-Aggf1) (5 μg kg−1), and isoflurane + rh-Aggf1 (10 μg kg−1). qPCR and western blot assays were applied to detect the correlation between the expression of AGGF1 and isoflurane administration. Then, the Morris water maze (MWM) test was applied to evaluate the effect of AGGF1 on improving the POCD rats. Subsequently, TUNEL assay was applied and the cell apoptosis-related proteins were tested to reveal the anti-apoptotic effect of AGGF1 in POCD rats. Furthermore, the mRNA and protein levels of TNF-α, IL-6, and IL-1β were also detected by qPCR and ELISA to verify the anti-inflammatory effects of AGGF1 on POCD rats. Besides, the protein expression levels of PI3K, Akt, and NF-κB in each group were examined by western blot.

Results

In this study, the results revealed that isoflurane induced a decrease in AGGF1 expression in the hippocampus of aged rats. In addition, exogenous AGGF1 attenuated POCD in aged rats. Meanwhile, exogenous AGGF1 had anti-apoptotic and anti-inflammatory effects in POCD rats. Further research indicated that AGGF1 activated the PI3K/Akt pathway.

Conclusion

AGGF1 has neuroprotective effect against isoflurane-induced cognitive dysfunction in aged rats via activating the PI3K/AKT signaling pathways.

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Abstract  

The catalytic effects of iron, aluminum or silicon on the formation of NOX precursors (HCN, NH3 and HNCO) and HCl during wheat straw pyrolysis were studied using a thermogravimetric analyzer (TG) coupled with a Fourier transform infrared (FTIR) spectrometer in argon atmosphere. The results show that the presence of iron, aluminum or silicon decreases conversion of straw-N into NH3 with the sequence of Fe > Si > Al. The iron or silicon addition suppresses N-conversion into HCN and HNCO, and the aluminum addition has no notable influence on HCN emission during pyrolysis. The share of N-conversion to NH3 and HCN increases, but that to HNCO and NO decreases a little in the presence of added iron, aluminum or silicon. The addition of SiO2 results in the highest HCl removal efficiency.

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