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Abstract  

The extraction of cobalt by Winsor II microemulsion system was studied. In the bis (2-ethylhexyl) sulfosuccinate sodium salt (AOT)/n-pentanol/n-heptane/NaCl system, AOT was used as a anionic surfactant to form microemulsion in n-heptane, n-pentanol was injected in the microemulsion as a cosurfactant. Co(II) was found to be extracted into the microemulsion phase due to ion pair formation such as Co2+(R–SO3 )Cl. The influence of different parameters such as the volume ratio of aqueous phase to microemulsion, surfactant concentration, pH of the feed solutions, cosurfactant concentration as well as temperature on the extraction yield (E%) were investigated. The results showed that it was possible to extract 95% of cobalt by the AOT Winsor II microemulsion.

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Summary

An efficient and robust method for analyzing constituents of a well-known traditional Chinese medicine (TCM) formula SiWu decoction (SWD) contains Angelicae Sinensis Radix (ASR), Chuanxiong Rhizoma (CR), Paeoniae Radix Alba (PRA), and Rehmanniae Radix Praeparata (RRP) by ultra high-performance liquid chromatography (UHPLC)/time-of-flight mass spectrometry (TOF-MS) was established. The method efficiently applied to the separation of 75 compounds, including organic acids, phthalides, phenylpropanoid glycosides, iridoid glycosides, monoterpene glycosides, and galloyl glycosides in the complex prescription, 52 compounds in this study can be unambiguously identified or tentatively characterized. The separation was achieved within 20 min at the optimized chromatographic conditions. Our study provided a reliable and high-efficient method for the understanding of the chemical basis of SWD.

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In this study, the cDNA of homocysteine S-methyltransferase was isolated from Aegilops tauschii Coss., with the gene accordingly designated as AetHMT1. Similar to other methyltransferases, AetHMT1 contains a GGCCR consensus sequence for a possible zinc-binding motif near the C-terminal and a conserved cysteine residue upstream of the zinc-binding motif. Analysis of AetHMT1 uncovered no obvious chloroplast or mitochondrial targeting sequences. We functionally expressed AetHMT1 in Escherichia coli and confirmed its biological activity, as evidenced by a positive HMT enzyme activity of 164.516 ± 17.378 nmol min−1 mg−1 protein when catalyzing the transformation of L-homocysteine. Compared with the bacterium containing the empty vector, E. coli harboring the recombinant AetHMT1 plasmid showed much higher tolerance to selenate and selenite. AetHMT1 transcript amounts in different organs were increased by Na2SeO4 treatment, with roots accumulating higher amounts than stems, old leaves and new leaves. We have therefore successfully isolated HMT1 from Ae. tauschii and characterized the biochemical and physiological functions of the corresponding protein.

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Journal of Thermal Analysis and Calorimetry
Authors: J. Zhi, W. Tian-Fang, L. Shu-Fen, Z. Feng-Qi, L. Zi-Ru, Y. Cui-Mei, L. Yang, L. Shang-Wen, and Z. Gang-Zhui

Abstract  

The effects of aluminum (Al) and nickel (Ni) powders of various grain sizes on the thermal decomposition of ammonium perchlorate (AP) were investigated by TG and DSC in a dynamic nitrogen atmosphere. The TG results show that Al powders have no effect on the thermal decomposition of AP at conventional grain size, while the nanometer-sized Ni powders (n-Ni) have a great influence on the thermal decomposition of AP with conventional and superfine grain size. The results obtained by DSC and an in situ FTIR analysis of the solid residues confirmed the promoting effects of n-Ni. The effects of n-Ni have been ascribed to its enhancement on the gas phase reactions during the second step decomposition of conventional grain size AP.

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