We obtain the structure theorem for -Hopf bimodules over Hopf algebroids, where H is the total algebra of the Hopf algebroid . Based on this theorem, we investigate the structure theorem for comodule algebras over Hopf algebroids.
The thermogenic curves of metabolism of the four strains of Escherichia coli pUC19cab/JM109, pUC19cab/XL-IBlue, JM109 and XL-IBlue were determined using the LKB-2277 BioActivity Monitor and the ampoule
method at 37�C. The pUC19cab/JM109 and pUC19cab/XL-IBlue are recombinant E. coli strains bearing the same foreign plasmid pUC19cab, which confers the ability to produce polyhydroxyalkanoates (PHAs). The
yield of PHAs of pUC19cab/XL-IBlue was higher than that of pUC19cab/JM109. XL-IBlue and JM109 were the host bacteria. The
heat flow of these strains was XL-Blue≈JM109>pUC19cab/JM109>pUC19cab/XL-IBlue. These results indicate an obvious interrelation
between the PHAs production and the heat flow rate of E. coli strains.
The paper is concerned with endomorphism algebras for weak Doi-Hopf modules. Under the condition “weak Hopf-Galois extensions”,
we present the structure theorem of endomorphism algebras for weak Doi-Hopf modules, which extends Theorem 3.2 given by Schneider
in . As applications of the structure theorem, we obtain the Kreimer-Takeuchi theorem (see Theorem 1.7 in ) and the
Nikshych duality theorem (see Theorem 3.3 in ) in the case of weak Hopf algebras, respectively.
Radioiodination of tri-n-butylstannyl-3-quinuclidinyl benzilate (TQNB) and N-succinimidyl-3-(tri-n-butylstannyl) benzoate (STB) was studied. STB was radiolabeled efficiently using iodogen to prepare radioactive N-succinimidyl-3- iodobenzoate (S125IB). TQNB was radioiodinated using Chloramine-T to obtain radioactive iodo-3-quinuclidinyl benzilate (125IQNB). Both S125IB and 125IQNB showed good stability at room temperature in the dark.
Authors:Y. Wang, L. Yin, G. Lv, Y. Xu, L. Xu, Y. Qi, L. Zheng, and J. Peng
In the present paper, a simple and reliable high-performance liquid chromatography-diode array detection (HPLC-DAD) method was developed both for quantitative determination and fingerprint analysis of Agrimonia pilosa Ledeb for quality control. Under the optimized HPLC conditions, seven bioactive compounds including rutin, quercetin-3-rhamnoside, luteoloside, tiliroside, apigenin, kaempferol, and agrimonolide were determined simultaneously. For fingerprint analysis, 11 common peaks were selected as the characteristic peaks to evaluate the similarities of 16 different samples collected from different origins in China. Besides, hierarchical cluster analysis (HCA) was also performed to evaluate the variation of the raw materials. This is the first report of using a simple method for quality control of A. pilosa Ledeb through multi-component determination and chromatographic fingerprint analysis to the best of our knowledge.
Synthesis, characterization and thermal analysis of polyaniline (PANI)/ZrO2 composite and PANI was reported in our early work. In this present, the kinetic analysis of decomposition process for these
two materials was performed under non-isothermal conditions. The activation energies were calculated through Friedman and
Ozawa-Flynn-Wall methods, and the possible kinetic model functions have been estimated through the multiple linear regression
method. The results show that the kinetic models for the decomposition process of PANI/ZrO2 composite and PANI are all D3, and the corresponding function is ƒ(α)=1.5(1−α)2/3[1−(1-α)1/3]−1. The correlated kinetic parameters are Ea=112.7±9.2 kJ mol−1, lnA=13.9 and Ea=81.8±5.6 kJ mol−1, lnA=8.8 for PANI/ZrO2 composite and PANI, respectively.
Authors:Y. Zhang, X. An, X. Li, S. Chen, L. Gao, K. Wang, S. Wang, and Y. Yan
Two new y-type HMW-GSs in
with the mobility order of 1Dy12.2
>1Dy12, were identified by both SDS-PAGE and MALDI-TOF-MS. Molecular cloning and sequencing showed that the genes encoding subunits 1Dy12.1*
had identical nucleotide acid sequences with 1,947 bp encoding a mature protein of 627 residues. Their deduced molecular weights were 67,347.6 Da, satisfactorily corresponding to that of 1Dy12.2
subunit determined by MALDI-TOF-MS (67,015.7 Da), but was significantly smaller than that of the the 1Dy12.1*
subunit (68,577.1 Da). Both subunits showed high similarities to 1Dy10, suggesting that they could have a positive effect on bread-making quality. Interestingly, the expressed protein of the cloned ORF from accessions TD87 and TD130 in
co-migrated with subunit 1Dy12.2
, but moved slightly faster than 1Dy12.1*
on SDS-PAGE. The expressed protein in transgenic tobacco seeds, however, had the same mobility as the 1Dy12.1*
subunit, as confirmed by both SDS-PAGE and Western blotting. Although direct evidence of phosphoprotein could not be obtained by specific staining method, certain types of post-translational modifications (PTMs) of the 1Dy12.1*
subunit could not be excluded. We believe PTMs might be responsible for the molecular weight difference between the subunits 1Dy12.1*
Authors:B. Peng, P. Li, S. Lai, Y. Wang, L. Yang, and Y. Wang
High ozone (O3) can cause great damage to plants. However, the effect of high O3 on nitrogen (N) absorption, distribution, and utilization in rice at different growth stages under different planting densities is poorly understood. In the present study, a conventional cultivar (Yangdao 6) and a hybrid cultivar (II You 084) with different planting densities were exposed to an elevated amount of O3 (E-O3; 50% higher than that of the control, C-O3) under a freeair gas concentration enrichment (FACE) system. N absorption, distribution, and utilization of the green leaves, stems, and shoots at tillering, jointing heading, and maturity were investigated. Results showed that E-O3 significantly increased the N content in the shoots of Yangdao 6 by 7.5%, 12.7%, and 19.6%, respectively, at jointing, heading, and maturity. Also, the N content in the shoots of II You 084 increased by 5.4%, 6.5%, and 8.4% at the corresponding growth stage upon E-O3 application. E-O3 significantly decreased N accumulation of II You 084 by 8.3%, 4.9%, 4.7%, and 19.2%, respectively, at tillering, jointing, heading, and maturity. Further, E-O3 had a decreasing effect on the N distribution in green leaves (p ≤ 0.05) of both cultivars, but exerted an increasing effect on that in the stems of both cultivars (p ≤ 0.05). In addition, E-O3 significantly decreased the N use efficiency (NUE) for biomass of the two cultivars in all growth stages. These results revealed that E-O3 could increase the N content in rice plants but decrease the N accumulation and utilization in both cultivars. The effects of E-O3 on N absorption, distribution, and utilization were not affected by planting density.
Authors:Y. Duan, J. Li, X. Yang, X. Cao, L. Hu, Z. Wang, Y. Liu, and C. Wang
The thermal decomposition of strontium acetate hemihydrate has been studied by TG-DTA/DSC and TG coupled with Fourier transform
infrared spectroscopy (FTIR) under non-isothermal conditions in nitrogen gas from ambient temperature to 600°C. The TG-DTA/DSC
experiments indicate the decomposition goes mainly through two steps: the dehydration and the subsequent decomposition of
anhydrous strontium acetate into strontium carbonate. TG-FTIR analysis of the evolved products from the non-oxidative thermal
degradation indicates mainly the release of water, acetone and carbon dioxide. The model-free isoconversional methods are
employed to calculate the Ea of both steps at different conversion α from 0.1 to 0.9 with increment of 0.05. The relative constant apparent Ea values during dehydration (0.5<α<0.9) of strontium acetate hemihydrate and decomposition of anhydrous strontium acetate (0.5<α<0.9)
suggest that the simplex reactions involved in the corresponding thermal events. The most probable kinetic models during dehydration
and decomposition have been estimated by means of the master plots method.
Authors:W. N. Zhou, J. Ouyang, Z. H. Wang, X. Y. Wang, Y. R. Suo, Z. Zhang, and H. L. Wang
Elsholtzia densa Benth. var. densa (Lamiaceae) is a famous medicinal herb which has been widely used for treatment of colds, headaches, pharyngitis, fever, diarrhea, digestion disorder, rheumatic arthritis, nephritises, and nyctalopia in China. In this study, fraction of the ethyl alcohol extract of E. densa (aerial part) by different polarity solvents indicated that the ethyl acetate soluble fraction exhibited a potent 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity with the IC50 value of 148.2 μg/mL. Under the target guidance of DPPH experiment, isoquercitrin, trachelogenin, ethyl caffeate, and arctigenin were separated with purities 95.98%, 92.98%, 96.07%, and 88.83%, respectively, by a dual-mode high-speed counter-current chromatography (HSCCC) method using n-hexane–ethyl acetate–methanol–water (4.5:5:3:4, v/v/v/v) as the solvent system. In order to evaluate the scientific basis, antioxidant activity of four isolated compounds was assessed by the radical scavenging effect on DPPH radical; isoquercitrin and ethyl caffeate showed stronger antioxidant activities with IC50 values of 9.4 μg/mL and 9.2 μg/mL, respectively, while trachelogenin and arctigenin showed weak antioxidant activities with IC50 values of >500 μg/mL and 72.8 μg/mL, respectively. Results of the present study indicated that the combinative method using DPPH antioxidant assay and dual-mode HSCCC could be widely applied for rapid screening and isolating of antioxidants from complex traditional Chinese medicine extract.