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  • Author or Editor: Y.W. Wang x
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Abstract  

N,N,N,N-tetrabutylsuccinylamide (TBSA) was synthesized, characterised and used for the extraction of U(VI) and Th(IV) from nitric acid solutions into n-dodecane. Extraction distribution coefficients of U(VI) and Th(IV) as a function of aqueous nitric acid concentration, extractant concentration and temperature have been measured and found that n-dodecane as diluent was superior to 50% 1,2,4-trimethyl benzene (TMB) and 50% kerosene, (OK) system for extraction of U(VI) and Th(IV). The compositions of extracted species, equilibrium constants and enthalpies of extraction reactions have also been calculated. The formation of the 121 complex of uranyl(II) ion or the 141 complex of thorium(IV) ion, nitrate ion and TBSA and extracted species was further confirmed by the IR spectra of saturated extract of U(IV) and Th(IV).

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Abstract  

The complex (C11H18NO)2CuCl4(s) was synthesized. Chemical analysis, elemental analysis, and X-ray crystallography were used to characterize the structure and composition of the complex. Low-temperature heat-capacities of the compound were measured by an adiabatic calorimeter in the temperature range from 77 to 400 K. A phase transition of the compound took place in the region of 297–368 K. Experimental molar heat-capacities were fitted to two polynomial equations of heat-capacities as a function of the reduced temperature by least square method. The peak temperature, molar enthalpy, and entropy of phase transition of the compound were calculated to be T trs = 354.214 ± 0.298 K, Δtrs H m = 76.327 ± 0.328 kJ mol−1, and Δtrs S m = 51.340 ± 0.164 J K−1 mol−1.

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Fructose-bisphosphate aldolase (FBA, EC 4.1.2.13) catalyzes an aldol cleavage of fructose-1, 6-bisphosphate to dihydroxyacetone-phosphate and glyceraldehyde 3-phosphate and a reversible aldol condensation. Three candidate genes with 1077bp coding for fructose-bisphosphate aldolase were cloned and sequenced in wheat, barley and rye. These genes could encode 358 amino acid residues. Sequence analysis indicated that wheat, barley and rye FBA genes were conserved with high identity (94.13%), while maize sequence had a 9bp deletion near the 3’ terminal. According to the alignment of 75 amino acid sequences, conserved domains of the FBAs were detected. These conserved domains might be the important functional sites of the FBAs. The cytoplasmic FBAs of wheat, barley and rye were clustered together, and the cluster was close to maize and rice FBAs. Nine peptides of the FBAs and the last amino acid Tyr (necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate) were most conserved in plants, animals and algae. Current findings suggested that the FBAs could be divided into three main subgroups: plant cytoplasmic FBA, plant chloroplastic FBA and animal FBA. These results also indicated that the active and binding sites of FBAs had rare variations during the long-term evolution.

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A comparative proteomic analysis of grain proteins during five grain developmental stages of wheat cultivar Chinese Spring (CS) and its 1Sl/1B substitution line CS-1Sl(1B) was carried out in the current study. A total of 78 differentially expressed protein (DEP) spots with at least 2-fold expression difference were detected by two-dimensional electrophoresis (2-DE). Among these, 73 protein spots representing 55 differentially expressed proteins (DEPs) were successfully identified by matrix-assisted laser desorption/ionization time-offlight tandem mass spectrometry (MALDI-TOF/TOF-MS). Differential protein spots between the two genotypes were analyzed by cluster software, which revealed significant proteome differences. There were 39 common spots (including 33 DEPs) that showed significant difference between the two lines across five grain developmental stages, of which 14 DEP spots (including 11 DEPs) were mainly involved in carbohydrate metabolism that were encoded by the genes on 1B chromosome while 25 DEP spots (including 12 DEPs) were mainly related to stress response and gluten quality that were encoded by 1S1 chromosome. These results indicated that the Sl genome harbors more stress and quality related genes that are potential valuable for improving wheat stress resistance and product quality.

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Abstract  

A microcalorimeter (Setaram c-80) was used to study the thermokinetics of the hydration process of calcium phosphate cement (CPC), a biocompatible biomaterial used in bone repair. The hydration enthalpy was determined to be 35.8 J g–1 at 37.0°C when up to 80 mg CPC was dissolved in 2 mL of citric buffer. In the present study, parameters related to time constants of the calorimeter were obtained by fitting the recorded thermal curves with the function θ=Ae–?t(1– e–?2t). The real thermogenetic curves were then retrieved with Tian function and the transformation rate of the hydration process of CPC was found to follow the equation α=1–[1–(0.0075t)3]3. The microstructures of the hydrated CPC were examined by scanning electron microscopy. The nano-scale flake microstructures are due to crystallization of calcium phosphate and they could contribute to the good biocompatibility and high bioactivity.

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Abstract  

The extraction of europium to a W/O microemulsion with an anionic surfactant was studied. In the sodium oleate (NaOL)/pentanol/heptane/NaCl system, the influence of aqueous-microemulsion ratio, concentration of NaOL, extraction temperature, concentration of cosurfactant, pH and salting-out agent on the extraction yield were investigated. Europium was probably extracted into the microemulsion phase in the form of Eu(OL)2Cl, and the extraction yield (E%) was above 99% when R = 8. The enthalpy and entropy of Eu(III) extraction were calculated to be −12.18 kJ/mol and −61.41 J/(mol K), respectively. The back-extraction is conducted by hydrochloric acid (0.8 mol/L), which provided better back-extraction yields (95.15%).

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Summary

A simple and rapid method, using online ultraperformance liquid chromatography with photodiode array detection and electrospray ionization mass spectrometry (UPLC-PDA-eλ-ESI-MS/MS), was developed for the in-depth analysis of 50 batches Radix et Rhizoma Rhei. The analysis was performed on a UPLC BEH C18 column using a gradient elution system. Baseline separation could be achieved in less than 7.5 min. At the same time, on the basis of the 50 batches of samples collected from representative cultivated regions, a novel chromatographic fingerprint was devised by UPLC-PDA, in which 27 common peaks were detected and identified by the developed UPLC-MS/MS method step by step according to fragmentation mechanisms, MS/MS data, standards, and relevant literature. Many active components gave prominent [M - H] ions in the ESI mass spectra. These components include anthraquinones, sennosides, stilbenes, glucose gallates, naphthalenes, and catechins. Furthermore, based on the information of these Radix et Rhizoma Rhei components, and further combined with discriminant analysis, a novel discriminant analysis equation (DAE) was established for the quality control of Radix et Rhizoma Rhei for the first time.

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Summary

A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of glycyrrhizin, formononetin, glycyrrhetinic acid, liquiritin, isoliquiritigenin, and licochalcone A in licorice. An Eclipse Plus C18 column (I.D. 4.6 × 100 mm, 3.5 μm particle size; Agilent) was used in the analysis. Electrospray ionization (ESI)-tandem interface in the negative mode was performed, and multiple reaction monitoring (MRM) was employed with the precursor multiple reaction monitoring production combination for the determination of six analytes. The average recoveries ranged from 98.30% to 100.13% with relative standard deviations (RSDs) ≤ 1.95%, and limits of detection (LODs) ranged from 2.1 to 3.6 pg. The applicability of this analytical approach was confirmed by the successful analysis of six samples. The results indicated that the established method was validated, sensitive, and reliable for the determination of six analytes in licorice.

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Abstract  

Organic peroxides (OPs) have caused many momentous explosions and runaway reactions, resulting from thermal instability, chemical pollutants, and even mechanical shock. In Taiwan, dicumyl peroxide (DCPO), due to its unstable reactive nature, has caused two thermal explosions and runaway reaction incidents in the manufacturing process. To evaluate thermal hazards of DCPO in a batch reactor, we studied thermokinetic parameters, such as heat of decomposition (†H d), exothermic onset temperature (T 0), maximum temperature rise ((dT/dt)max), maximum pressure rise ((dP/dt)max), self-heating rate (dT/dt), etc., via differential scanning calorimetry (DSC) and vent sizing package 2 (VSP2).

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Elsholtzia densa Benth. var. densa (Lamiaceae) is a famous medicinal herb which has been widely used for treatment of colds, headaches, pharyngitis, fever, diarrhea, digestion disorder, rheumatic arthritis, nephritises, and nyctalopia in China. In this study, fraction of the ethyl alcohol extract of E. densa (aerial part) by different polarity solvents indicated that the ethyl acetate soluble fraction exhibited a potent 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity with the IC50 value of 148.2 μg/mL. Under the target guidance of DPPH experiment, isoquercitrin, trachelogenin, ethyl caffeate, and arctigenin were separated with purities 95.98%, 92.98%, 96.07%, and 88.83%, respectively, by a dual-mode high-speed counter-current chromatography (HSCCC) method using n-hexane–ethyl acetate–methanol–water (4.5:5:3:4, v/v/v/v) as the solvent system. In order to evaluate the scientific basis, antioxidant activity of four isolated compounds was assessed by the radical scavenging effect on DPPH radical; isoquercitrin and ethyl caffeate showed stronger antioxidant activities with IC50 values of 9.4 μg/mL and 9.2 μg/mL, respectively, while trachelogenin and arctigenin showed weak antioxidant activities with IC50 values of >500 μg/mL and 72.8 μg/mL, respectively. Results of the present study indicated that the combinative method using DPPH antioxidant assay and dual-mode HSCCC could be widely applied for rapid screening and isolating of antioxidants from complex traditional Chinese medicine extract.

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