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Acta Veterinaria Hungarica
Authors:
Zong-Xi Cao
,
Pei-Rong Jiao
,
Yu-Mao Huang
,
Hong-Yang Qin
,
Liu-Wu Kong
,
Quan-Hui Pan
,
Yi-Min He
, and
Gui-Hong Zhang

To understand the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in South China, we collected 231 clinical samples from pigs with suspected PRRSV infection in Guangdong between 2007 and 2009. We found that 74 of 231 samples were positive by RT-PCR. The PCR products of the ORF5 gene of 35 isolates from different farms were sequenced and their DNA sequences were compared to 23 other PRRSV isolates in the GenBank. We found that the nucleotide similarity among all South China isolates ranged from 87.6% to 100%, and all belonged to the North American genotype. Most of them were classified into subgenotype I, but the rest mapped to subgenotypes III, V or VI. Those in subgenotypes I and III were found to be highly variable in the primary neutralising epitope (PNE) with a specific amino acid mutation (F39/L39→I39), and a few isolates in subgenotypes I and III isolates also had a mutation at L41 (L41→S41). PRRSV isolates in subgenotypes III, V and VI had less potential glycosylation sites than those in subgenotype I. Our data contribute to the understanding of molecular variation of PRRSV in South China.

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Thermal dissociation reactions and mechanism of complexes of rare earth(iii) nitrates with the crown ether benzo-15-crown-5 were investigated by means of TG-DTG, DSC, DTA and IR technique. The results have shown that the dissociation processes of the complexes consist of several steps, one of which is a fast decomposition reaction. The fast decomposition peak temperatures (DSC) of all the complexes of the lanthanides (except Pm and Tm) decrease regularly with increasing atomic number. Moreover, values of the enthalpy change of desolvation, fast and the fourth step of decomposition and the apparent activation energies of fast and the fourth step of decomposition were obtained.

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