Authors:Yun Sang Cho, Sang Eun Lee, Youngboo Jang, Sukchan Jung and Jong Man Kim
Bovine tuberculosis (bTB) is a common zoonosis prevalent in many countries with grave economic consequences. Most developed and developing countries have implemented the test-and-slaughter policy to protect public health and reduce economic losses in the cattle industry. The official diagnosis of bTB is based on assays dependent on cell-mediated immunity (CMI). CMI-based diagnosis demonstrates diagnostic incapability at late stages of infection, which could be overcome by diagnosis based on humoral immunity (HI). Therefore, there is an urgent need to identify and define the B cell antigenome of Mycobacterium bovis. In this study, the B cell antigenome of culture filtrate proteins (CFP) was defined by mass spectrometry-based proteomics technology. Four spots were detected on 2-dimensional gel electrophoresis (2-DE) against M. bovis-positive serum in an immunoblotting experiment. Twenty-one proteins were identified in four spots by proteomic tools, such as Mb2900, Mb2898, Mb0448, Mb3834c, Mb1918c, Mb0134c, Mb0358 and Mb1868c, which are known B cell antigens, including 13 new proteins, i.e. Mb3751, Mb2006c, Mb3276c, Mb2244, Mb1164c, Mb2553c, Mb2946c, Mb1849c, Mb1511c, Mb1034c, Mb2616c, Mb0854c and Mb2267. These new proteins identified by 2-DE and immunoblotting were the B cell antigens used in developing serological diagnostic methods based on HI to bTB.
Authors:Choon-ho Cho, Yun-sang Lee, Eung-soo Kim, Jeong-guk Kim and Han-soo Lee
The uranium ingot casting process is one of the steps which consolidate uranium deposits produced by electrorefiner in an
ingot form in a pryprocessing technique. Since molten uranium metal reacts with a graphite crucible when the uranium is being
dissolved, a graphite crucible cannot be used. Accordingly, a ceramic material must be selected which does not react with
the dissolving uranium and this must be used as a coating material on the graphite crucible surface. As to this research,
a reactivity experiments were performed between the coating layer and uranium by applying a thermal spray coating to the graphite
material with alumina and YSZ ceramic material. As shown in the experimental result, the YSZ coating layer showed a stronger
adhesive property on the side where there is no Ni–Al binding material. Moreover, no reaction was apparent between the YSZ
coating layer and the uranium. Accordingly, the YSZ material and the process of thermal spray coating are considered to solve
the reactive problem between uranium and a graphite crucible.