The loss of lead in various kinds of biological materials after drying and ashing is studied by using203Pb as tracer which is intraveneously injected into mice. The experimental results show that although the oven ashing can be used for determining the lead in samples of lung, kidney, and liver, etc., the oxygen plasma and high pressure digestion are the most suitable methods for the determination of lead in biological materials.
A new thermokinetic reduced extent method for the product inhibition of single substrate enzyme-catalyzed reactions is proposed
and compared with the traditional initial rate method in this paper. The arginase-catalyzed hydrolysis of L-arginine to L-ornithine and urea was studied at 37C in 40 mM sodium barbiturate-HCl buffer solution (pH=9.4). Michaelis constant (Km) for arginine and maximum velocity (Vm) of the reaction were determined by initial method and thermokinetic method. The activation of exogenous manganese to this
reaction was also studied. The product inhibition constant (KP), which cannot be obtained directly from the initial rate method, was determined by thermokinetic without adding L-ornithine
to the reaction system. When the concentration of Mn2+ in cell is 0.1 mM, the enzyme gets its full activity. Incubation arginase with appropriate concentration of Mn2+resulted in increased Vmax and a higher sensitivity of the enzyme to product with no change in the Km for arginine. We suggest that the exogenous manganese ions in solution have just recovered the activity of arginase, which
was lost in dissolving and dilution, but no effect on the mechanism of the reaction.
The dehydration process of Co(II), Cu(II)
and Zn(II) methanesulfonates was studied by thermogravimetry/derivative thermogravimetry
(TG/DTG) and differential scanning calorimetry (DSC) techniques in dynamic
N2 atmosphere. The TG/DTG curves show that all of them
contain four crystallization water molecules, which are lost in two steps.
The peak temperature and dehydration enthalpies ΔH
were measured from DSC curves for each compound. The effect of procedural
variables on the TG and DSC curves was investigated. In this work, the procedural
variables included heating rate, Al pan state (unsealed and sealed) and sample
Positron annihilation lifetime spectra and ionic conductivity have been measured for poly(etherurethane)-LiClO4 as a function of temperature. The effects of Li salt on glas transition free volume and ionic conductivity have been discussed. A correlation between fractional free volume and ionic conductivity was first experimentally confirmed by using the free volume theory.
Authors:X. Cao, Z. Wang, X. Yang, Y. Liu and C. Wang
The kinetics of protein thermal transition is of a significant interest from the standpoint of medical treatment. The effect
of sucrose (0–15 mass%) on bovine serum albumin denatured aggregation kinetics at high concentration was studied by the iso-conversional
method and the master plots method using differential scanning calorimetry. The observed aggregation was irreversible and
conformed to the simple order reaction. The denaturation temperature (Tm), the kinetic triplets all increased as the sucrose concentration increased, which indicated the remarkable stabilization
effect of sucrose. The study purpose is to provide new opportunities in exploring aggregation kinetics mechanisms in the presence
As a new form of traditional Chinese medicine (TCM), Zuojin pill extract is made containing Coptidis rhizoma (the rhizome of Coptis chinesis Franch. [Ranunculaceae]) and Euodiae fructus (the unripe fruit of Euodia rutaecarpa [Juss.] Benth. [Rutaceae]) at the ratio of 6:1 (w/w) and has been most widely used in TCM to treat gastro-intestinal disorders. However, the quality control is insufficient. For establishing an analysis method for the effective quality control of Zuojin pill extract, the active components of three protoberberine alkaloids (coptisine, palmatine, berberine, components from C. rhizoma) and two indolequinoline alkaloids (evodiamine, rutaecarpine, components from E. fructus) in Zuojin pill extract were determined simultaneously by high-performance liquid chromatography. Chromatographic separations were performed on an Inertsil ODS-3 column (4.6 mm × 250 mm, 5 μm). Elution was carried out at 25°C under isocratic conditions by using 25 mmol L−1 KH2PO4-25 mmol L−1 SDS-acetonitrile (1:1:2, v/v/v, adjusted pH to 3.0 using phosphoric acid) as a mobile phase with a flow rate of 0.5 mL min−1. Detection wavelength was set at 264 nm. Good linearity was obtained with correlation coefficient higher than 0.999 for all the analytes over the investigated concentration ranges. Three batches of Zuojin pill extract were successfully analyzed. The average percentages of coptisine, palmatine, berberine, evodiamine, and rutaecarpine in Zuojin pill extract were 5.35%, 3.92%, 16.64%, 0.03%, and 0.03%, respectively. Consequently, these protoberberine and indolequinoline alkaloids could be identified and determined easily by the established high-performance liquid chromatography (HPLC) method which can be used to evaluate and control the quality of Zuojin pill extract.
Authors:Ping Zhou, Z. Wang, Y. Xu, Lei Zhang and Y. Wang
Uranyl luminescences in phosphoric acid system has been studied. Uranyl excited by a nitrogen laser shows single or biexponential luminescence decays in the phosphoric acid system. When the uranyl ion or phosphoric acid concentration are lower, a single exponential luminescence decay appears, whereas at higher uranyl ion or phosphoric acid concentrations, biexponential decay is observed. Time-resolved spectra of uranyl in this system are measured. The reasons of the phenomena are tentatively established.
Two methods, e.g. initial rate method and thermokinetic reduced extent method were presented for studies on non-competitive
inhibition. Arginase-catalyzed the hydrolysis of L-arginine toL-ornithine and urea and the inhibition of this reaction by sodium fluoride were studied in the absence and presence of exogenous
of Mn2+at 37C in 40 mM sodium barbiturate-hydrochloric acid buffer solution (pH 7.4). Both methods were successively used to determine
the values of K1. The advances and disadvantages of each method were compared in this paper. Exogenous Mn2+ could result in more sensitivity of arginase to F-1. Since the inhibition of arginase by F-1 depends on the pH values of the reaction system and behave as a non-competitive inhibition, it probably due to its small
volume and high electronic density allow it access to the activity site of the enzyme and replaces of -OH2 (or -OH) as the bridge ligand with Mn(II, II) cluster. However, further studies are necessary to determine the modes of
interaction of F-1 with bovine liver arginase.
The interactions of lanthanide ions (Ln3+) with bovine serum albumin (BSA) under mimetic physiological conditions (310.15 K, pH 6.7, 0.1MNaCl) were studied by microcalorimetry.
For the first time, based on Two Sets of Independent Sites Model, molar enthalpies (ΔrHm1, ΔrHm2) and coordination number (n1, n2) of the two sets of binding sites with different affinity were obtained directly from the microcalorimetric results. It was
shown that the interactions are endothermic and entropy-driving processes. By combining with fluorescence spectroscopy, other
thermodynamic parameters (ΔrGm1, ΔrSm1) were determined for high-affinity specific sites.
The radiochemical method has been used for investigation of the adsorption of radium on eighteen inorganic ion exchangers. The distribution coefficient of radium obtained are as follows: barite 2955, celestite 2420, BaSO4 4350, BaCrO4 5245, Ba3(PO4)2 5775, MnO2·nH2O 1681, La2O3·nH2O 4150, Zerolit S/F 2920, etc.