The depth profile of phosphorus concentration in phosphorus-doped silicon was shown to be measureable with an accuracy of 1–3% by the use of liquid scintillation counting after neutron irradiation. Both the amount of Si and P are determined by neutron activation analysis followed by liquid scintillation counting for 1–5 hours after the end of irradiation and 5–10 days after irradiation, respectively. Concentrations as low as 10 ppm and thicknesses of 20 nm can be determined.
The kinetics of thermal decomposition of a series of uranyl nitrate complexes with N-alkylcaprolactams (alkyl=C2H5, C4H9, C6H13, C8H17, C10H21 or C12H25) was studied by means of non-isothermal gravimetry under a nitrogen atmosphere. From the TG-DTG curves, the kinetic parameters
relating to the loss of two molecules of coordinated ligand were obtained by employing two groups of methods: (I) a group
of conventional methods involving the Coast-Redfern, Freeman-Carroll, Horowitz-Metzger, Dharwadkar-Karkhanavala and Doyle
(modified by Zsakó) equations; (II) a new method were suggested by J. Máleket al.. The results obtained using two types of methods were compared, and it emerged that the results of method II were much more
meaningful and reasonable in this work. Additionally, the effects of the molecular structure of the ligands on the kinetic
data and models were studied and are discussed.
Authors:Z. Zeng, T. Zhang, G. Li, C. Liu, and Z. Yang
A synthetic autopolyploid was developed from diploid Aegilops tauschii, D genome progenitor of common wheat (Triticum aestivum). The tetraploid Ae. tauschii displayed a markedly larger organ size than the diploid donor. Fluorescence in situ hybridization (FISH) and DNA marker analysis revealed that there is no clear variation at either the chromosomal or DNA level between the diploid and tetraploid plants. We analyzed the variation in cytosine methylation patterns between the diploid and tetraploid plants by methylation-sensitive amplified polymorphism (MSAP) and detected 228 and 232 methylated sites in diploid and tetraploid plants, respectively. Statistical comparison indicated that the tetraploid Ae. tauschii genotype displayed no significant difference in polymorphic methylation level compared to the diploid ones. Twenty-two different genomic fragments displaying different methylation behavior during the ploidy conversions were isolated and sequenced. It demonstrated that alterations in the level of methylation have the most profound effects on coding genes. We demonstrated that there are some genes expressions modified by DNA methylation may be correlated with phenotypic alteration after autotetraploidization.
Authors:X. He, Z. Zhang, L. Feng, Z. Li, J. Yang, Y. Zhao, and Z. Chai
After an acute exposure to lanthanum chloride, the pharmacokinetics of calcium uptake in rats was studied by radioactive 47Ca tracer. The accumulated doses of calcium in the left femurs during 24 hours were determined. The results showed that the
area under the curves (AUC), specific activity of maximal blood 47Ca concentration (Cmax), distribution rate constant (Ka) and the accumulated dose of calcium in the left femur decreased while time to Cmax (Tpeak) increased with the rising dosage of lanthanum exposure. It indicated that lanthanum expose had a negative effect on calcium
Authors:Z. Tang, S. Fu, Z. Ren, H. Zhang, Z. Yang, B. Yan, and H. Zhang
Wheat-rye 1BL.1RS translocations have been widely used in wheat breeding programs. A 1BL.1RS translocation wheat line, 91S-23, was developed from a 1R monosomic addition of the rye
inbred line L155 into wheat
MY11. A new commercial wheat cultivar, CN18, which also contained the 1BL.1RS translocation, was derived from the cross MY11 × 91S-23. Polymerase chain reaction (PCR) and fluorescence
hybridization (FISH) indicated that the rye centromere was eliminated from the 1BL.1RS chromosomes of CN18 but not from 91S-23. Based on the 1RS source and the centromeric structure of the translocation chromosome, CN18 qualifies as a new wheat cultivar possessing a 1BL.1RS translocation. CN18 displayed high yield performance and resistance to powdery mildew and stripe rust, whereas 91S-23 was susceptible to these diseases. The present study provides a new 1RS resource for wheat improvement.
Authors:X. Cao, Z. Wang, X. Yang, Y. Liu, and C. Wang
The kinetics of protein thermal transition is of a significant interest from the standpoint of medical treatment. The effect
of sucrose (0–15 mass%) on bovine serum albumin denatured aggregation kinetics at high concentration was studied by the iso-conversional
method and the master plots method using differential scanning calorimetry. The observed aggregation was irreversible and
conformed to the simple order reaction. The denaturation temperature (Tm), the kinetic triplets all increased as the sucrose concentration increased, which indicated the remarkable stabilization
effect of sucrose. The study purpose is to provide new opportunities in exploring aggregation kinetics mechanisms in the presence
Authors:J. Jia, G. Li, C. Liu, M. Lei, and Z. Yang
Wheat yellow rust resistance gene Yr17 was originated from the wheat-Aegilops ventricosa introgression, and still effective on the adult plant in Southern China. The previous studies located the gene Yr17 on the translocation of 2NS-2AS using the molecular and cytological markers. In the present study, we screened new PCR-based markers to map the gene Yr17 region from the investigation of a segregating 120 F2 population. All markers including four EST-PCR markers, a SCAR (sequence characterized amplified region) and a PLUG (PCR based landmark unique gene) marker specific to Yr17 gene were mapped on the chromosome 2AS, and located on the chromosomal deletion bin 2AS5-0.8–1.00 region. Based on the wheat-rice collinearity, we found that the sequences of the Yr17 gene linked markers were comparatively matched at rice chromosome 4 and chromosome 7. However, the identified closely linked genomic sequence of Yr17 gene is most likely collinear with genomic region of rice chromosome 4. The newly produced PCR based markers closely linked to Yr17 gene will be useful for the marker-assisted selection in wheat breeding for rust resistance.