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The antagonistic effect of thirteen Pseudomonas aeruginosa and thirteen strains of other Pseudomonas species was studied on the soil-borne phytopathogenic Rhizoctonia solani and Fusarium solani fungi.  The inhibition of pathogen colony growth was tested with two different in vitro techniques using the same type of culture media. In case of the spread slant technique the antagonists induced a significantly stronger inhibition on the growth of pathogens than in case of spot transfer. Among the 26 investigated Pseudomonas strains, P. aeruginosa strains were generally more effective against the fungal pathogens. Rhizoctonia solani proved to be affected to a greater extent by the bacterial strains studied than the Fusarium solani representative. The possibility of in vitro strain selection of biocontrol microbes is being further discussed .

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Eighteen isolates of fluorescent pseudomonads ad Bacillus spp. were isolated from the Meloidogyne incognita suppressive soils of tomato fields. These isolates were evaluated in the laboratory and green house for the biocontrol of M. incognita . Eight isolates were considered to have potential for the biocontrol of M. incognita on the basis of the antibiotic sensitivity, fluorescence produced by Pseudomonas , inhibitory effect on the hatching and penetration of M. incognita and root colonization of tomato root by these isolates. These 8 isolates (Pa22, Pf25, Pf27, Pa28, B22, B23, B27 and B28) were further tested for their biocontrol potential against M. incognita on tomato in a pot test. Out of 8 isolates, isolate B28 was the best in improving tomato growth of M. incognita inoculated plants. Isolate B28 also caused greater reductions in galling and multiplication of M. incognita on tomato while isolate Pa28 was found best in improving growth of plants without M. incognita .

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Literature Amin , F. , Razdan , V. , Bhat , K. and Banday , S. ( 2010 ): Potential of Trichoderma species as biocontrol agents of soil borne fungal propagules . J. Phytology 2 , 38 – 41 . Balasuriya , A. and Kalaichelvan

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76 60 65 Chet, I. (1987): Trichoderma application, mode of action and potential as a biocontrol agent of soil-borne plant pathogenic fungi. In

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Baker, R. and Dickman, M. B. (1993): Biocontrol with fungi. - In: Blaine Metting, F. Jr. (ed.): Soil microbial ecology - application in agricultural and environmental management. Marcer Dekker Inc., New York, pp. 27

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60 575 581 Stephan, D., Schmitt, A., Carvalho, S.M., Seddon, B. and Koch, E. (2005): Evaluation of biocontrol preparations and plant extracts for

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Trichoderma sp. capable of chestnut blight biocontrol. FEMS Microbiol Lett 126 , 249 (1995). Randomly amplified polymorphic DNA fingerprinting identifies subgroups of Trichoderma viride and other Trichoderma sp. capable of

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geographical locations of Chhattisgarh in Central India. SpringerPlus 1, 73. 2 Benítez, T., Rincón, A. M., Limón, M. C. and Codón, A. C. (2004): Biocontrol

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biocontrol agents: some case studies. Can. J. Plant Pathol. 23 , 8–12. Bélanger R. R. Approaches to molecular characterization of fungal biocontrol agents: some case studies

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. Biocontrol Science and Technology 13, 403–413. Corley J. C. The functional response of parasitoids and its implications for biological control Biocontrol

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