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and K. pneumoniae strains by means of PCR using the primer sets and thermal cycling conditions described in Tables  I and II . PCR products were analyzed by electrophoresis in a 1%–1.5% agarose gel. One of the PCR products was purified and direct

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Acta Microbiologica et Immunologica Hungarica
Authors: Enikő Fehér, Szilvia Marton, Ádám György Tóth, Krisztina Ursu, Kerstin Wernike, Martin Beer, Ádám Dán and Krisztián Bányai

-PCR method (primer sequences and protocol available upon request). Fragments of the SBV S and M segments were amplified using the primer sets designed by Fischer et al. [ 5 ], whereas PCR products covering the complete L segment were generated by new

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gene due to its strong recommendation by Coker and Davies [ 22 ]. A primer set used for studying α-Tubulin expression was the same as used by Xu and Shi [ 23 ], at their recommended thermocyclic conditions in GeneAmp-2700 thermocycler (Applied

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Acta Microbiologica et Immunologica Hungarica
Authors: Seyedeh Marzieh Jabbari Shiadeh, Ali Hashemi, Fatemeh Fallah, Parnian Lak, Leila Azimi and Marjan Rashidan

). PCR amplification of efrA and efrB genes The presence of efrA and efrB genes was detected by PCR using the sequence-specific primer sets described in Table  I . PCR was carried out in a total volume of 25 μL Master mix 2× (Sinaclon

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(annealing temperature for each primer set is shown in Table  I ), and extension (72 °C for 1 min), and a final extension of 5 min at 72 °C. Ten microliters of reaction mixture containing PCR product was analyzed by gelelectrophoresis in 0.8% (wt/vol) agarose

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E. coli -harboring ESBLs and AmpC β-lactamases by PCR technique (Bio Intellectica PCR). The primer sets and thermal cycling conditions described in Tables  I and II . One of the PCR products was purified and direct sequencing was performed. Two

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buffer and used for PCR amplification. To detect the major SE genes ( sea , seb , sec , sed , and see ), multiplex PCR protocols were performed using the primer sets of the European Union Reference Laboratory for Coagulase-Positive Staphylococci (EURL

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pGem ® -T Vector System (Promega, Madison, USA) according to manufacturer’s instructions. Insert sequences were amplified using M13 primer sets [ 42 ] followed by nested PCR reaction with the applied specific primers (27F-519R, A340F-A934R, amoA –F1

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European Journal of Microbiology and Immunology
Authors: Markus Krohn, Thomas Wanek, Marie-Claude Menet, Andreas Noack, Xavier Declèves, Oliver Langer, Wolfgang Löscher and Jens Pahnke

photometrically. cDNA was synthesized using the High Capacity RNA-to-cDNA Kit (Applied Biosystems, USA). Gene expression was analyzed with qRT-PCR using TaqMan Hybridization Probes. Primer sets with according TaqMan probes for Abcb1a (Mm00440761_m1), human ABCB

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Acta Microbiologica et Immunologica Hungarica
Authors: Mehrdad Mohammadi, Jila Yavarian, Vajihe Karbasizade, Sharareh Moghim, Bahram Nasr Esfahani and Nafiseh Sadat Hosseini

-Quraishy , S. A. , El Kholy , A. A. : A novel primer set for improved direct gene sequencing of human bocavirus genotype-1 from clinical samples . J Virol Methods 228 , 108 – 113 ( 2016 ). 10.1016/j

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