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Reverse Transcription Kits, USA). Using specific primer sets ( Table 1 ), aliquots of cDNA were amplified by a PCR machine (Peqlab, Germany), with initial denaturation at 94 °C for 5 min, followed by 33 cycles of denaturation at 94 °C for 45 s, annealing

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gel at 100 V for 60 min and were staining with safe stain. Table I. Primer sets used in this study

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Acta Veterinaria Hungarica
Authors: Mohsen Bashashati, Zohreh Mojahedi, Ali Ameghi Roudsari, Morteza Taghizadeh, Aidin Molouki, Najmeh Motamed, Fereshteh Sabouri and Mohammad Hossein Fallah Mehrabadi

editor and analysis program for Windows 95/98/NT . Nucleic Acids Symp. Ser. 41 , 95 – 98 . Hoffmann , E. , Stech , J. , Guan , Y. , Webster , R. G. and Perez , D. R ( 2001 ): Universal primer set for the full-length amplification of all

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Acta Microbiologica et Immunologica Hungarica
Authors: Orsolya Nagy, Anna Nagy, Szilvia Tóth, Bernadett Pályi, Anita Vargáné Koroknai and Mária Takács

primer sets specific for the 269 nt sequence of the NS3 and NS4A overlapping region of the viral genome (primer sequences are the following: first-round forward primer: CTGGCTTGAAGCAAGAATGC; first-round reverse primer: GGTCTCTAGGGTCTCCGGCA; second

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