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The relative efficiency of in vitro and in vivo screening techniques for drought tolerance, comprising various parameters, namely germination (%), shoot length, root length, coleoptile length, root number, root/shoot ratio and seedling vigour index (SVI) under in vitro conditions and morpho-physiological and yield-contributing traits under in vivo conditions, was studied using 78 winter × spring wheat-derived doubled haploid lines of bread wheat along with 13 parental genotypes and two check varieties, HPW 155 and PBW 343. Analysis of variance for different in vitro parameters in control (0 MPa) and stress (−0.7 MPa) environments and various in vivo parameters under irrigated and rainfed environments indicated sufficient genetic variability and the differential response of the genotypes to the different stress levels for all the in vitro and in vivo parameters. Correlation studies revealed the significance of percentage germination, root number, coleoptile length and seedling vigour index under in vitro conditions and relative water content and excised leaf water loss under in vivo conditions as important selection criteria for drought tolerance, as these parameters were related with each other as well as with the drought susceptibility index (S). The significant positive rank correlation between the in vitro (−0.7 MPa) and in vivo (rainfed) stress conditions indicated that the performance of a genotype under field conditions is very similar to its performance under laboratory conditions. Hence, the selection precision for a crucial and complex trait like drought tolerance in wheat can be enhanced by exercising in vitro selection coupled with evaluation in the field. The drought susceptibility index ‘S’ should not be taken as the sole criterion to categorize genotypes as drought-tolerant or susceptible ones.

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-genome chromosomes from Triticum aestivum produced with auto-allohexaploid triticale. Cereal Res. Commun. , 19 , 469-476. Tetraploid triticale with D-genome chromosomes from Triticum aestivum produced with auto-allohexaploid triticale

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Hungarian Medical Journal
Authors: Erika Csonka, Imre Cserpán, Katalin Fodor, Katalin Bajnóczky, István Andó, Gyula Hadlaczky and Györgyi Bujdosó

428 Bunnell, B. A., Izadpanah, R., Ledebur, H. C. Jr. et al.: Development of mammalian artificial chromosomes for the treatment of genetic diseases: Sandhoff and

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946 947 Carlton, P. M. (2008): Three-dimensional structured illumination microscopy and its application to chromosome structure. Chromosome Res. , 16 , 351

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Galiba, G., Quarrie, S. A., Sutka, J., Morgounov, A., Snape, J. W. (1995): RFLP mapping of vernalization (Vrnl) and frost resistance (Frl) genes on chromosome 5A of wheat. Theor. Appl. Genet. , 90 , 1174

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chromosomal aberrations, I. Utilisation of neurospora end nuclease for the study of aberration production in G-2 stages of cell cycle. Mutation Res., 1978, 52 , 137. Obe G. Molecular

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.: Chromosomal localization of mouse satellite DNA. Science 168 , 1356-1358 (1970) Chromosomal localization of mouse satellite DNA Science 168 1356 1358 Harper, M. E., Ullrich, A., Saunders, G. R.: Localisation of human insulin gene

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Belyayev, A., Raskina, O., Nevo, E. 2001. Chromosomal distribution of reverse transcriptase-containing retroelements in two Triticeae species. Chromosome Res. 9 :129

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Darlington, C. D., La Cour, L. E. (1976) The Handling of Chromosomes. 6th ed. Allen and Unwin, London. The Handling of Chromosomes

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