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Abstract  

A microcalorimetric technique based on the bacterial heat-output was explored to evaluate the effect of Mn(II) on Bacillus thuringiensis. The power-time curves of the growth metabolism of B. thuringiensis and the effect of Mn(II) on it were studied using an LKB-2277 BioActivity Monitor, ampoules method, at 28C. For evaluation of the results, the maximum peak-heat output power (P max) in the growth phase, the growth rate constants (k), the log phase heat effects (Q log ), and the total heat effect in 23 h (Q T) for B. thuringiensis were determined. Manganese has been regarded as the essential biological trace element. Mn(II) of different concentration have different effects on B. thuringiensis growth metabolism. High concentration (800-1600 μg mL-1) of Mn(II) can promote the growth of B. thuringiensis; low concentration (500-800 μg mL-1) can inhabit its growth.

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Abstract  

The thermogenic curves of the aerobic metabolism of the three strains of Bacillus thuringiensis B.t. A, B.t. B and B.t. C have been determined by using an LKB-2277 BioActivity Monitor. B.t. A was the host bacterium without foreign gene. B.t. B and B.t. C were constructed by transforming different foreign genes into the host B.t. A, respectively. B.t. B expressed erythromycin resistant gene, while B.t. C expressed both erythromycin resistant gene and tyrosinase gene. The heat flow rate of these strains is B.t. A> B.t. B >B.t. C. These results indicated that there is obvious interrelation between expression of foreign genes and heat flow rate of B.t. strains.

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in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin. Appl. Soil Ecol. 2 . 111-124. Changes in levels, species and DNA

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Journal of Thermal Analysis and Calorimetry
Authors: Yumei Han, Dan Yan, Yanling Zhao, Cheng Peng and Xiaohe Xiao

Pharmacol . 2006 ; 22 : 121 – 127 . 10.1016/j.etap.2005.12.011 . 6. Hayakawa , T , Howlader , MT , Yamagiwa , M , Sakai , H . Design and construction of a synthetic Bacillus

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