Authors:J. Yao, Y. Liu, Z. Gao, P. Liu, M. Sun, S. Qu and Z. Yu
A microcalorimetric technique based on the bacterial heat-output was explored to evaluate the effect of Mn(II) on Bacillus thuringiensis. The power-time curves of the growth metabolism of B. thuringiensis and the effect of Mn(II) on it were studied using an LKB-2277 BioActivity Monitor, ampoules method, at 28C. For evaluation
of the results, the maximum peak-heat output power (Pmax) in the growth phase, the growth rate constants (k), the log phase heat effects (Qlog ), and the total heat effect in 23 h (QT) for B. thuringiensis were determined. Manganese has been regarded as the essential biological trace element. Mn(II) of different concentration
have different effects on B. thuringiensis growth metabolism. High concentration (800-1600 μg mL-1) of Mn(II) can promote the growth of B. thuringiensis; low concentration (500-800 μg mL-1) can inhabit its growth.
Authors:L. Ruan, Y. Liu, Z. Gao, P. Shen and Q. Sheng
The thermogenic curves of the aerobic metabolism of the three strains of Bacillus thuringiensisB.t. A, B.t. B and B.t. C have been determined by using an LKB-2277 BioActivity Monitor. B.t. A was the host bacterium without foreign gene. B.t. B and B.t. C were constructed by transforming different foreign genes into the host B.t. A, respectively. B.t. B expressed erythromycin resistant gene, while B.t. C expressed both erythromycin resistant gene and tyrosinase gene. The heat flow rate of these strains is B.t. A> B.t. B >B.t. C. These results indicated that there is obvious interrelation between expression of foreign genes and heat flow rate of
in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillusthuringiensis var. kurstaki endotoxin. Appl. Soil Ecol. 2 . 111-124.
Changes in levels, species and DNA