Authors:K. Hoenes, M. Hess, P. Vatter, B. Spellerberg and M. Hessling
Saccharomyces cerevisiae (DSM no. 70449) was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany) and cultivated in a yeast extract–peptone–glucose (YEPG) medium. The medium consisted of 10 g yeast extract, 20 g
Authors:Omolbanin Jahantigh, Farzaneh Najafi, Hassanali Naghdi Badi, Ramazan Ali Khavari-Nejad and Forough Sanjarian
The relationships between salt stress and antioxidant enzymes activities, proline, phenol and anthocyanine contents in Hyssopus officinalis L. plants in growth stage were investigated. The plants were subjected to five levels of saline irrigation water, 0.37 (tap water as control) with 2, 4, 6, 8 and 10 dSm−1 of saline water. After two months the uniform plants were harvested for experimental analysis. Antioxidant enzymes activities and proline, phenol and anthocyanine contents of the plants were examinated. Enhanced activities of peroxidase, catalase and superoxide dismutase were determined by increasing salinity that plays an important protective role in the ROS-scavenging process. Proline, phenol and anthocyanine contents increased significantly with increasing salinity. These results suggest that salinity tolerance of Hyssopus officinalis plants might be closely related with the increased capacity of antioxidative system to scavenge reactive oxygen species and with the accumulation of osmoprotectant proline, phenol and anthocyanine contents under salinity conditions.
Authors:Samira Samea-Andabjadid, Kazem Ghassemi-Golezani, Safar Nasrollahzadeh and Nosratollah Najafi
This research was conducted in a greenhouse to evaluate the effects of exogenous application of salicylic acid (SA) (1 mM) and 6-benzylaminopurine (BAP) (50 μM) on physiological performance of faba bean (Vicia faba) under different levels of NaCl salinity (0, 4, 8 and 12 dS/m). The experiment was arranged as factorial on the bases of randomized complete block design in three replications. Leaf Na+ content, root and leaf soluble sugars, antioxidant enzymes activities such as catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and lipid peroxidation increased, but K+, K+/Na+ and membrane stability index (MSI) decreased as a result of salt stress. However, foliar sprays of BAP and particularly SA reduced Na+ content and lipid peroxidation, while enhanced the K+ content, K+/Na+, soluble sugars, antioxidant enzymes activities and MSI under different levels of salinity. It was, therefore, concluded that exogenous application of these growth regulators (GR) can considerably improve salt tolerance and physiological performance of faba bean.
Authors:M. Küchler, K. Ecker, E. Feldmeyer-Christe, U. Graf, H. Küchler and L.T. Waser
The detection and evaluation of changes in vegetation patterns is a prerequisite for monitoring programs. The Swiss mire monitoring program aims to assess the changes in mire vegetation in order to examine the efficiency of the management measures. A promising way to explore and detect vegetation structure and vegetation change is the application of predictive vegetation mapping that combines image classification and predictive habitat distribution models. These models deal with predictor variables derived from remotely sensed spectral data and from environmental variables such as a digital surface model (DSM). Low accuracy of environmental data to predict vegetation at the local scale is due to the difficulties to capture dominant fine-scale enironmental gradients. Using high resolution spectral and topographical data sets of 50 cm pixel size and below, the study presented here aims to improve the simulation of local-scale vegetation properties.
Wheat is the second most important crop after rice in India and occupies approximately 28.5 million hectare area. Salinity is one of the major factors reducing plant growth and productivity worldwide, and affects about 7% of world’s total land area. In India about 6.73 million hectare land area is salt affected. The aim of this study was to investigate the morpho- physiological and biochemical response of wheat to temporal salinity (ECiw = 10.0 dSm–1) exposures. Ten wheat genotypes were evaluated in two successive growing seasons (2012–2014), with complete randomized design with three replications under both salinity stress and non-stress conditions. The morpho-physiological and biochemical character measured in this investigation, inhibited under both salt stresses (S1 & S2) conditions but much more significantly inhibited under long-term salinity exposure (S2) than S1 because interrupting the metabolic process of plant, resulting in reduced growth and productivity. According to correlation result, selection of high yield genotypes can be done based on plant height (0.649*), tiller plant–1 (0.808**) and leaf area (0.687*). The multivariate morphophysiological and biochemical parameters should be further used to develop salinity tolerance in wheat breeding improvement programmes.
In the present investigation, expression of genes related to Na+ exclusion such as salt overly sensitive (TaSOS1) and Na+/H+ antiporter (TaNHX1) and proline accumulation such as pyrroline-5-carboxylate reductase (P5CR) and glutamate synthase (GOGAT) was studied in seedlings of Kharchia 65 (Kh 65, salt tolerant) and HD 2009 (sensitive) under salt stress (ECe, 12 dSm–1) and controlled conditions. As compared to HD 2009, Kh 65 showed significantly lower accumulation of Na+ (p < 0.01) and higher accumulation of proline (p < 0.05) in leaf blade under salt stress. The relative expression of TaSOS1 increased significantly (p < 0.001) in roots of Kh 65 (4.31-fold) while it decreased in HD 2009. There was significantly higher (p < 0.01) relative expression of TaNHX1 (27.57-fold) in leaf and 3.07-fold in roots of Kh 65 as compared to 3.65- and 0.87-fold increase in leaf and roots of HD 2009, respectively, under salt stress. There was significantly (p < 0.05) higher accumulation of proline as compared to HD 2009 in leaf tissues. There was significantly higher (p < 0.01) expression of P5CR (5.23-fold in leaf and 8.77-fold in the root) and glutamate synthase (6.0- fold in roots) in Kh 65 as compared to HD 2009. The study demonstrated that upregulation of genes for Na+ exclusion in root and compartmentation in leaf and increased proline concentration are associated with tolerance to salinity stress in wheat. The information will be useful for improving wheat genotypes for salt tolerance.
Authors:Christian Zimmermann, Marius Schild, Clemens Kunz, Kurt Zimmermann and Sabine Kuntz
E. coli S2-G1 (DSM 16441), S2-G3 (DSM 16443), S2-G4 (DSM 16444), and S2-G8 (DSM 16448). All bacterial strains were provided by SymbioPharm GmbH (Herborn, Germany). The strains are part of the probiotic Symbioflor® 2 (DSM 17252) drug. For incubation
(DSM 17252) could be injected into the bloodstream of mice without any deleterious effects [ 26 ]. These experiments were performed to demonstrate that these E. coli cells preferentially colonize cancer tissue, a characteristic that can actually have