A simple and sensitive method of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the determination of icariin in capsules by precolumn chelation with aluminum. In order to obtain a stable fluorescence signal, the reaction conditions of the fluorescent chelation complex between icariin and aluminum were investigated in detail. Chromatography was carried out on an Agilent Zorbax Extend C18 column (150 mm × 4.6 mm, 5.0 μm) using methanol as mobile phase at a flow rate of 1.0 mL min−1. The excitation and emission wavelengths were set at 430 and 480 nm, respectively. At optimum conditions, the calibration curve was linear in the concentration range from 0.010 to 100.0 μg mL−1 with the limit of detection of 3.5 ng mL−1 (S/N = 3). A comprehensive method was validated for precision and accuracy. The method described here has been successfully applied for the determination of the icariin content in a capsule with satisfactory results.
A simple, rapid, and sensitive reversed-phase HPLC method was developed and validated for determination of metronidazole and tinidazole in human plasma samples under identical chromatographic conditions. This method involves liquid-liquid extraction using chloroform: isopropylalcohol (95:5). Chromatographic separation was performed using a μ-bondapack C18 (250 mm × 4.6 mm) column. The mobile phase consisted of potassium dihydrogen phosphate solution (0.005 M)/acetonitrile (80/20 v/v). The final pH of the mobile phase was adjusted to 4 ± 0.1 with orthophosphoric acid. The calibration curves were linear over the concentration range 0.1–15 μg/mL for metronidazole and tinidazole with the detection limit of 30 ng/mL. Within- and between-day precision and accuracy did not exceed 9.83% and 10.48%, respectively. Metronidazole and tinidazole were found to be stable in plasma samples with no evidence of degradation during 3 freeze-thaw cycles and 3 months storage in −70 °C. The current validated bio-analytical method was finally applied in bioequivalence studies of two different metronidazole and tinidazole products according to a standard two-way cross-over design with a two-week washout period. No statistically significant difference was observed between the logarithmically transformed AUC0-∞ and Cmax values. Therefore, generic products were considered bioequivalent with those of standards which could be used interchangeably.
.030 ± 0.001 m%). These results imply that the object is covered with corrosion products, especially the rivets and their vicinity. Table 1. PGAA results of the dagger (Inv. Nr. 2013.10.03.) from Ráksi find in mass percent (m%) unit. Sb (detection limit: 0
. Forschungen in Tell-Siedlungen an Donau und Theiß. Budapest.
Menze, B. H.–Ur, J. A.–Sherratt, A. G. 2006 Detection of Ancient Settlement Mounds: Archaeological Survey Based on the SRTM Terrain Model. Photogrammetric Engineering
(‘music’), while lágy (‘soft’) and puha (‘tender’) are synonyms in the context of margarin (‘margarine’) and fém (‘metal’). Our main objective in the present proof-of concept experiment is the detection of adjectival cliques of this type: cliques
satellites as reference points for geodesic surveys and computers to speed up the recording and processing of survey data. The use of the Global Positioning System (GPS). as well as of Light Detection and Ranging (LiDAR) 3D laser scanners have aided in
. This also highlights a potential limitation when using screen recording in and of itself as a diagnostic protocol for error detection in this context. We can readily see and articulate on errors in conjunction with on-screen phenomena that occur prior
.02 1.04 0.17 1.06 0.20 1.71 0.54 0.11 Std dev 0.12 0.05 0.02 0.20 0.01 0.02 0.01 0.04 0.03 0.02 0.04 0.02 0.05 0.03 0.02 0.05 0.05 0.02 Due to the detection limits of the SEM-EDS technique, the reliable quantification is not possible for a number of
eredményeiről (Metal detection investigations at Eastern Hungarian Late Bronze Age and Early Iron Age sites. Report on the results of the bronze hoard exploration project of the Institute of Archaeology of ELTE in 2009) . Kisfaludi J. (szerk.): Régészeti
dataset will be extended with the corpus occurrences of complex forms listed above. However, in this initial phase, even the accurate detection of simple forms poses a great challenge which is mainly due to their rather free distribution relative to the