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Acta Biologica Hungarica
Authors: Eszter László, P. Kiss, Gabriella Horváth, P. Szakály, Andrea Tamás, and Dóra Reglődi

Arimura, A., Somogyvári-Vigh, A., Miyata, A., Mizuno, K., Coy, D. H., Kitada, C. (1991) Tissue distribution of PACAP as determined by RIA: highly abundant in the rat brain and testes. Endocrinology 129 , 2787

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Acta Biologica Hungarica
Authors: Ádám Simon, János Oláh, István Komlósi, András Jávor, József Németh, Zoltán Szilvássy, Dóra Reglődi, Andrea Tamás, and Levente Czeglédi

, A. , Borzsei , R. , Bagoly , T. , Kiss , P. , Horvath , G. , Brubel , R. , Nemeth , J. , Szalontai , B. , Szabadfi , K. ( 2011 ) Presence of pituitary adenylate cyclase-activating polypeptide (PACAP) in the plasma and milk of

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Magyar Sebészet
Authors: Klára Nedvig, Györgyi Szabó, Domokos Csukás, József Sándor, József Németh, Krisztina Kovács, Dóra Reglődi, Ágnes Kemény, György Wéber, and Andrea Ferencz

2009 Influence of PACAP on oxidative stress and tissue injury following small bowel autotransplantation J Mol Neurosci 37 168 76

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European Journal of Microbiology and Immunology
Authors: Markus M. Heimesaat, Gernot Reifenberger, Viktoria Vicena, Anita Illes, Gabriella Horvath, Andrea Tamas, Balazs D. Fulop, Stefan Bereswill, and Dora Reglodi

functions . Pharmacol Rev 52 , 269 – 324 ( 2000 ) 3. Gomariz RP , Juarranz Y , Abad C , Arranz A , Leceta J , Martinez C : VIP-PACAP system

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Introduction Pituitary adenylate cyclase activating polypeptide (PACAP) was first isolated from ovine hypothalamic extracts. Based on the structural homology (68%) of its N-terminal amino acid sequence with the vasoactive intestinal peptide (VIP) it

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Journal of Radioanalytical and Nuclear Chemistry
Authors: J. Németh, B. Jakab, R. Józsa, T. Hollósy, A. Tamás, A. Lubics, I. Lengvári, P. Kiss, Zs. Oberritter, B. Horváth, Z. Szilvássy, and D. Reglődi

Abstract  

Pituitary adenylate cyclase activating polypeptide (PACAP) is a widely distributed neuropeptide that has two molecular forms with 38 and 27 amino acid residues. The aim of the present study was to develop a new, highly specific PACAP-27 assay to investigate the quantitative distribution of PACAP-27 in the central nervous system of various vertebrate species applying the same technical and experimental conditions. Our results show that the antiserum used turned to be PACAP-27 specific. The average ID50 value was 51.5±3.6 fmol/ml and the detection limit was 2 fmol/ml. PACAP-27 immunoreactivity was present in the examined brain areas, with highest concentration in the rat diencephalon and telencephalon. Swine and pigeon brain also contained significant amount of PACAP-27. Our results confirm the previously described data showing that PACAP-38 is the dominant form of PACAP in vertebrates, since PACAP-38 levels exceeded those of PACAP-27 in all examined brain areas. Furthermore, our study describes for the first time, the comparative quantitative distribution of PACAP-27 and-38 in the swine and pigeon brain.

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Acta Physiologica Hungarica
Authors: K. Csanaky, D. Reglődi, E. Bánki, I. Tarcai, L. Márk, Zs Helyes, T. Ertl, J. Gyarmati, K. Horváth, L. Sántik, and Andrea Tamás

Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuropeptide with special importance in reproductive and developmental processes. PACAP is found in two bioactive forms: PACAP27 and PACAP38. Recently, we have described that PACAP38 is present in high levels in the milk of human and ruminant animals. Breastfeeding is of utmost importance in proper nutrition of the newborn, but artificial nursing with infant formulas is necessary when breastfeeding is not available. Composition of the breast milk varies during the whole period of nursing and it shows differences at the beginning (foremilk) and the end of an actual suckling (hindmilk). The aim of this study was to investigate PACAP38-like immunoreactivity (PACAP38-LI) in different milk and infant formula samples by radioimmunoassay and to prove the presence of PACAP38 in the infant formula by mass spectrometry. We found similar PACAP38-LI in human mature foremilk and hindmilk samples, in the fresh and pasteurized cow milk and also in formulas. However, we found significantly higher PACAP38-LI in the hypoantigenic formula undergoing extensive hydrolysis compared to the non-hypoantigenic ones. Our results suggest that PACAP38 is relatively stable in the milk and it can withstand the manufacturing processes.

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Introduction

It was previously shown that intracerebroventricular administration of pituitary adenylate cyclase-activating polypeptide (PACAP) prior to GnRH mobilization in proestrus prevents ovulation in rats. In this study, we examined whether PACAP given intranasally could influence luteinizing hormone (LH) and prolactin (PRL) surges and ovulation.

Methods

On the day of proestrus PACAP, β-cyclodextrin (modifier of blood–brain barrier) or PACAP + β-cyclodextrin was applied intranasally between 12:30 and 13:00. Blood samples were taken at 16:00, 18:00, and 20:00 for measuring plasma hormone levels. In the next morning, the expelled ova were counted. β-Cyclodextrin was also administered to male and diestrous female rats between 12:30 and 13:00 and blood was taken at 18:00.

Results

PACAP prevented LH and PRL surges and ovulation in about half of the rats, β-cyclodextrin alone more effectively prevented ovulation. When PACAP and β-cyclodextrin were administered together, more rats ovulated like when PACAP given alone. β-Cyclodextrin did not influence LH and PRL levels in diestrous females; however, in males, it significantly enhanced PRL level.

Discussion

Not only the intracerebroventricular, but the intranasal application of PACAP prevented ovulation. β-Cyclodextrin alone is more effective than PACAP and enhances PRL levels in male rats. PACAP and β-cyclodextrin given together weaken each other’s effect. β-Cyclodextrin, as excipient of various drugs, has to be used carefully in human medications.

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A “null-biopszia” klinikai értéke

Klinikai és kísérletes lehetőségek az átültethető vesék számának növelésére és minőségük javítására

Clinical value of “zero-hour biopsy”

Clinical and experimental opportunities of qualitative and quantitative improvement of renal transplantation
Magyar Sebészet
Author: Péter Szakály

PACAP: The road to discovery Peptides 28 1617 9 . 21. S Shioda

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by PACAP-immunoreactive fibers originating in the trigeminal ganglion: a degeneration study. Cell Tissue. Res. 301 , 369-373. Innervation of the rat pineal gland by PACAP-immunoreactive fibers originating in the trigeminal

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